T4/T8 ratio

CD4+/CD8+ cells play an important regulatory role in the immune response process. Clinically detecting the T lymphocyte subsets or TH/TS ratio in peripheral blood of patients, it is helpful to understand the immune status of the body and the pathogenesis and auxiliary diagnosis of certain diseases. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Tips: Try to encourage patients to take oral, choose a digestible high protein diet. Normal value 2 to 3:1. Clinical significance (1) Reduce tumor, viral infection, fungal infection, and immunodeficiency. (2) Elevating autoimmune and allergic diseases. Low results may be diseases: high results of combined immunodeficiency disease may be diseases: autoimmune hemolytic anemia considerations Same as B cell detection. In addition, due to the non-specific binding of sheep or rabbit anti-mouse fluorescent antibodies used in indirect immunofluorescence technology to the surface of some leukocytes, and the non-specific cellular reaction of Fc receptors and the amplification effect of polyclonal fluorescent antibodies, non-specific positive fluorescence is caused. Increased cells. For the FITC direct monoclonal antibody, the antibody composition, properties and structure are completely uniform, as long as the protein-FITC complex is not charged with excessive charge, no non-specific adsorption will occur. At present, foreign countries basically use direct immunofluorescence and flow cytometry to analyze T cell subsets. Due to the high price of the instrument, it has not been widely used in China. Therefore, for general clinical laboratories, this can also be done with an immunofluorescence microscope. Inspection process (1) Direct immunofluorescence: 1 Take heparin anticoagulated peripheral blood, obtain mononuclear cells with lymphocyte stratification solution, and Hank liquid is formulated into (1 ~ 2) × 106 cells / ml. 2 Take 1ml of cell suspension into a 5ml plastic tube, centrifuge 2000r / min, 2min, discard the supernatant. 3 Add 20 μl of each of FITC-CD4 and FITC-CD8, add mIgG-FITC to the control tube, and react at 40 °C for 30 min. 4Hank was washed twice, 2000r/min, 2min. 5 flow cytometry analysis. (2) Indirect immunofluorescence: 1 Isolation of PBMC, adjusted to (1 ~ 2) × 106 cells / ml. 2 Take 1 ml of the cell suspension in a 5 ml plastic tube, centrifuge at 2000 r/min for 2 min, and discard the supernatant. 3 Add 100 μl of each anti-CD4 and CD8 monoclonal antibody, add the antibody dilution or normal mouse IgG to the control tube, and react at 40 °C for 30 min. 4Hank was washed twice, centrifuged at 2000 r/min for 2 min, and the supernatant was discarded. 5 Each 50 μl of FITC-IgG was added and reacted at 4 ° C for 30 min. 6 Hank was washed twice, 2000 r/min, 2 min. 7 drops, fluorescence microscopy or flow cytometry analysis. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.

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