EA rosette activation assay

There are Fc receptors on the surface of B lymphocytes, which can bind to the Fc segment of immunoglobulin (lgG). Therefore, antibody-sensitized bovine erythrocytes or chicken red blood cells are mixed with B lymphocytes, and it is seen that B lymphocytes are adhered around B lymphocytes or Chicken red blood cells, after staining, it can be seen that red bovine red blood cells or chicken red blood cells and central blue lymphocytes form a rose petal shape, forming a wreath, which is called E garland, which is called E garland. Anyone with 3 or more bovine red blood cells or chicken red blood cells attached to the surface is called a wreath forming cell (ie, EA positive cells). 200 lymphocytes were counted under a microscope, and the percentage of rosette formation was calculated, and the percentage of B lymphocytes was estimated. Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: not fasting Tips: Actively cooperate with the doctor during the examination. Normal value EA-RFT 0.10 to 0.30 (10% to 30%). Clinical significance EA wreath value increased: Found in chronic lymphocytic leukemia, hairy cell leukemia, whooping cough, measles, chickenpox, leprosy, etc. EA wreath value reduction: Found in primary and secondary immunodeficiency diseases, malignant tumors, systemic lupus erythematosus, leprosy, viral infections, dermatomyositis and so on. Precautions The blood sample to be inspected should be fresh, otherwise the lymphocytes will die easily, thus affecting the formation rate of the wreath. Inspection process Take peripheral blood, use heparin anticoagulation (per milliliter of whole blood plus heparin 10~ smart unit without preservative), and add 1~2 times of cold Hanks balanced salt solution (HBSS) without calcium and magnesium ions. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.

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