tumor necrosis factor alpha

Tumor Necrosis Factor (TNF) can be divided into TNFα and TNFβ. The former is derived from mononuclear macrophages, the latter is produced by activated T cells, and is a cytokine with important biological activity. The TNF receptors present on the cells are mainly composed of two kinds of TNFRI and TNFRII, and soluble TNFR (sTNFRI, sTNFRII) is present in the serum. It was found in the 1980s to play an important role in the consumption of dyscrasia. TNF is mainly produced by activated macrophages, NK cells and T lymphocytes. Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: fasting Tips: Patients should avoid access to a variety of sources. Normal value The radioimmunoassay is 0.74 to 1.54 ng/ml. Clinical significance Abnormal results: The luciferase gene of the reporter plasmid of tumor necrosis factor alpha gene is driven by the tumor necrosis factor alpha gene promoter and the 3' untranslated sequence, and the expression level of luciferase gene reflects the expression of tumor necrosis factor alpha gene. Level. The reporter plasmid for the tumor necrosis factor alpha gene was used to examine the effect of the compound on the expression level of the tumor necrosis factor alpha gene. People who need to be examined: patients with liver inflammation, tissue inflammation, and abnormal blood sugar. Precautions Pre-inspection contraindications: Patients should avoid access to a variety of radioactive sources. Requirements for examination: The specimens were taken with 3 ml of venous blood at the time of diagnosis, and the serum was separated in time, and the concentration was determined by freezing at -40 °C. Inspection process Double antibody sandwich enzyme immunoassay. The TNFα monoclonal antibody is coated on the PVC plate, and the TNFα and the single antibody of the sample species are combined with the horseradish peroxidase-labeled anti-TNFα polyclonal antibody, and the peroxidase and the TMB are terminated and the enzyme label is used. The instrument measured the optical density at 450 nm, and the results were compared with the standard curve to determine the content of TNFα. Not suitable for the crowd Inappropriate crowd: None. Adverse reactions and risks There are no related complications and hazards.

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