Cellular immune check

Cellular immunoassays are involved in an immune response or a cellular examination associated with an immune response. These include lymphocytes, monocytes, macrophages, granulocytes, mast cells, helper cells, and their precursor cells. There are mainly lymphocyte transformation test, FBC rose test, T lymphocyte subset, lymphocyte toxicity test and NK cell activity assay. A method of detecting the number and function of various cells (i.e., immune cells) involved in an immune response. The number or function of immune cells can vary due to diseases of the immune system or other systems, or due to immunization or certain clinical treatments and certain environmental factors. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Tips: Blood specimens are generally blood samples in the morning on an empty stomach; fecal specimens are forbidden to eat meat and animal blood foods and banned from iron and vitamin C on the 3rd day before the test; semen specimens must be abstinent for 3 to 7 days before collection. Vaginal secretion specimens should be prohibited from intercourse, bathing, vaginal examination, vaginal lavage and topical medicine before collection. Normal value Generally negative results. Some tests are within a certain range of reference values. Clinical significance Abnormal results: 1. T lymphocytes can be used for T lymphocyte count, classification of T lymphocyte subsets, and determination of T lymphocyte activation. CD3 molecules are expressed on the surface of all mature T lymphocytes and are important markers of total T lymphocytes. CD3+ is common in hyperthyroidism, lymphocytic thyroiditis, myasthenia gravis, and rejection after organ transplantation. Mainly found in immunodeficiency diseases, such as AIDS, congenital thymic hypoplasia syndrome and combined immunodeficiency disease. Can also be seen in malignant tumors, SLE, immunosuppressive therapy. 2. The increase of SmIg+ cells in B lymphocytes is often associated with the malignant proliferation of B cells, mainly in chronic colitis, hairy cell leukemia and macroglobulinemia. SmIg+ cell reduction is mainly related to humoral immunodeficiency, which is common in gamma globulin deficiency and severe combined immunodeficiency disease. People who need to be examined: those with low immunity and those with impaired cellular immunity can be examined. Precautions Pre-inspection contraindications: blood specimens are generally blood samples in the morning fasting; urine specimens and blood specimens should pay attention to the effects of diet, exercise and drug volume; fecal specimens are forbidden to eat meat and contain 3 days before the test. Animal blood food and banned iron, vitamin C, etc.; cerebrospinal fluid specimens, chest and ascites specimens should be promptly sent for examination; prostatic fluid specimens should be collected after massage of the prostate; semen specimens should be abstinent for 3 to 7 days before collection; vaginal secretions Specimens should be prohibited from sexual intercourse, bathing, vaginal examination, vaginal lavage and topical medicine before collection. Requirements for examination: blood venous blood sampling should pay attention to hemolysis; urine specimens require aseptic operation; stool specimens should be kept for inspection; cerebrospinal fluid specimens should be promptly sent for inspection and inspection should pay attention to safety; chest and ascites specimens and The cerebrospinal fluid specimens are the same; the prostatic fluid specimens should be promptly sent for inspection and attention to prevent the specimens from being evaporated; the semen specimens should be kept warm and promptly sent for inspection; the vaginal secretion specimens should be noted that all instruments are clean. Inspection process T lymphocyte surface marker detection: E rose flower formation test, immunofluorescence (IFA), fluorescence activated cell sorting (FACS), immunogold and silver method, and immunoenzymatic staining. Lymphocyte function test: T cell transformation test using morphological method. B lymphocyte surface marker detection: B cell surface immunoglobulin (BCR, SmIg) is a characteristic surface marker of B cells. B cells can be divided into SmIgG, SmIgM, SmIgA, SmIgD, and SmIgE by detecting fluorescently labeled monoclonal antibodies against different types of Ig. Not suitable for the crowd Inappropriate people: There are generally no special requirements. Female patients undergoing menstruation should not be examined for specimens of vaginal secretions. Adverse reactions and risks No relevant information.

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