sperm agglutination test

The sperm agglutination test begins with the sperm agglutination test in gelatin originally founded by Kibrik et al. (1952), followed by the development of four gelatin agglutination tests (Kibrik, 1952); 2 test tube agglutination test (Franklin, Dukes, 1964); 3 capillary agglutination test (Shulman, Hekman, Pann, 1971); 4 shallow disk agglutination test (Friberg, 1974). Basic Information Specialist classification: male examination check classification: semen and prostatic fluid examination Applicable gender: whether the male is fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: The result of the negative test indicates that it should be normal. Positive: A positive test indicates that the subject may have sperm agglutination. Tips: Before leaving semen, the patient should stop sexual intercourse for 4 to 7 days. Testosterone propionate, testosterone phenylacetate, and nantrolone phenylpropionate could not be used 1 week before the test. Drinking should be stopped within 1 month before the test. This must be done. Normal value The sperm is evenly dispersed in the gelatin. If the black paper is used as the back side to observe the light, it is evenly milky white, and no agglomerate is negative. Clinical significance The abnormal result is that the sperm is a small agglomerate, and it can be seen from the light observation that the small agglomerate can be diagnosed as sperm agglutination like a snowflake. Infertile patients with sperm agglutination in the population to be examined. Positive results may be diseases: male infertility, male disease, immune infertility precautions Taboo before inspection: 1. Before leaving semen, the patient should stop sexual intercourse for 4 to 7 days. Testosterone propionate, testosterone phenylacetate, and nantrolone phenylpropionate could not be used 1 week before the test. Drinking should be stopped within 1 month before the test. This must be done. 2, when taking semen, soft soap or paraffin oil can be used for penile massage, the specimens are collected in sterile test tubes; condoms can be used (washed clean, no spermicide drugs) or semen can be collected by sexual intercourse, but this way The amount collected is often small. 3. If the semen is not obtained by the above method, the seminal vesicle and the end of the vas deferens can be massaged through the rectum, and the urine can be collected to check whether there is sperm in the sediment. 4. Do not expose semen to overheated and cold environments. Hand it to the doctor for reference, no more than 30 minutes. Keep warm in cold weather, and keep it in your underwear pocket when you send it. 5. If bacterial culture is to be carried out, the urethral opening should be rinsed and disinfected, and the semen should be collected in a sterile test tube. Requirements for inspection: Actively cooperate with doctors. Inspection process Gelatinagglutination test (GAT) 【ready】 (1) Formulating Baker solution (Bakersolution); Glucose 3.00g Na2HPO4·7H2O0.46g; NaCl0.20gKH2PO40.01g; Add double distilled water to 100 mL. (2) Prepare a 10% white gelatin solution with Baker solution, and place it in a 37 ° C water bath for use. (3) Sperm suspension: Take normal fresh semen (sperm density is about 80×106/mL, activity rate>60%, a and b active sperm >70%), liquefy at room temperature, dilute to 40× with Baker solution 106/mL, stored in a 37 ° C water bath for later use. (4) The serum to be tested and the control serum were treated by heating in a water bath at 56 ° C for 30 min to inactivate complement. Subsequently, both serums were diluted 1:4 to 1:32 with Baker solution, and stored in a 37 ° C water bath for use. 【method】 (1) Take a certain amount of sperm suspension, add an equal amount of 10% gelatin solution, and mix well, then add 0.3 mL to each small test tube containing 0.3 mL of double-diluted serum, and mix them thoroughly. (2) Dispense the mixture solution into another small test tube (3mm×30mm) with a capillary pipette, incubate at 37°C for 2h, and displace it at 4°C for 5min. After the gelatin is solidified, observe the sperm agglutination in the gelatin and judge the result. . [judgment] (1) Negative: The sperm is evenly dispersed in the gelatin. If the black paper is used as the back side to observe the light, it is evenly milky white, and no aggregates are seen. (2) Positive: The sperm is a small agglomerate, and it can be seen that the small agglomerates are like snow flakes. It is not difficult to identify. (3) Antibody titer: The highest serum dilution factor of the positive reaction is the serum agglutination antibody titer. Not suitable for the crowd The examination is less invasive and generally has no contraindications. Adverse reactions and risks This test is less invasive and generally does not cause serious complications or other hazards.

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