sperm acrosome enzyme

The acrosomal enzyme is present on the inner membrane of the sperm acrosome and on the equatorial membrane, usually in an inactive form. When the sperm head enters the zona pellucida, the acrosin is activated as acrosin. This enzyme is an indispensable neutral proteolytic enzyme in the process of fertilization. Its action is similar to that of trypsin. It can hydrolyze the egg zona pellucida, allowing sperm to pass through the cumulus and then through the zona pellucida, so that the sperm and egg are fused. It also promotes the release of kinins in the reproductive tract, thereby enhancing sperm motility and promoting sperm movement. Basic Information Specialist classification: male examination check classification: semen and prostatic fluid examination Applicable gender: whether the male is fasting: not fasting Analysis results: Below normal: Male infertility. Normal value: Sperm acrosome enzyme: 15.29-58.15U/L Above normal: Rare. negative: Positive: Tips: You need to abstain from sex for 3-7 days before the test. It is recommended to avoid drinking and overwork in the near future. Normal value (36.72 ± 21.43) U / L (BAEE-ADH method). Clinical significance Acrosin is indispensable for sperm movement and fertilization, and lack of acrosome enzyme activity can lead to male infertility. Therefore, the determination of sperm acrosome enzyme activity can be used as a reference indicator for sperm fertilization ability and diagnosis of male infertility. Low results may be diseases: precautions for male infertility Acrosin also promotes the release of kinins in the reproductive system, thereby enhancing sperm motility and promoting sperm movement. Insufficient acrosome enzyme activity can lead to male infertility. Inspection process (1) Washing of sperm and extraction of acrosin: Take 0.25 ml of liquefied semen in a plastic centrifuge tube and centrifuge at 500 × g for 5 min; discard the supernatant and invert the centrifuge tube on the absorbent paper to remove the remaining solution. Add 0.25ml of 2% acetic acid solution, put it in the refrigerator at 4 °C overnight (16 ~ 24h), remove it, centrifuge at 1000 × g for 10min, and take the supernatant for use. (2) Measurement procedure: 0.1 ml of NAD (1 mg/ml) freshly prepared with pH 8.70.05 mol/LTris-HCl buffer, BAEE 1 mg/ml 0.5 ml, ADH (3.46 mg/ml) 0.1 were sequentially added to the cuvette. 2.25ml of ml and Tris-HCl buffer, uniformly mixed and pre-warmed at 25 °C for 5min, then add 0.05ml of acrosome enzyme extract to mix immediately, record the change of absorbance of the reaction solution at 366nm 10min after adding enzyme solution. (△A366mm/10min). (3) Definition of enzyme activity unit and calculation of enzyme activity: 1 milliunit (mu) of acrosome enzyme activity is equal to BAEN of 1 nmol/L per minute under the above reaction conditions (pH 8.5 and 25 ° C) The amount of acrosome enzyme required. Since hydrolysis of 1 nmol/L of BAEE changes the absorbance of the reaction solution by 0.0011, the enzyme activity per ml of semen is calculated according to the following formula: t is the reaction time (10 min), ΔA is the change in absorbance in t minutes, and V is the volume of the enzyme solution (0.05 ml), so the enzyme activity According to the above formula, the enzyme activity per ml of the extract is obtained. Since the volume of the extract is the same as the volume of the original semen, the above formula calculates the acrosome activity per ml of semen. Not suitable for the crowd no. Adverse reactions and risks no.

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