Isolation and identification of fungi

The fungal is isolated and identified by separating and cultivating the fungus and then determining the strain according to the characteristics of the colony and the morphology of the microscopic. If necessary, biochemical reactions, identification tests, animal inoculation, etc., to identify the species. Strict aseptic operation to avoid contamination. During the cultivation period, if the growth of contaminated bacteria is found, it should be immediately transferred. On the second day after inoculation, day-to-day observations were made and growth was recorded. Positive culture can establish a diagnosis. Negatives need to be cultured for 3 weeks before they can report. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the body surface and the body flora are normal, and the human body is in a state of dynamic balance and health. Clinical significance Except for a few fungal cultures, most fungi can be cultured artificially. According to the appearance and microscopic characteristics of the colonies, the strains can be identified to make up for the lack of direct microscopy. Abnormal results Shallow fungi (flying fungi) only invade the skin, hair and nails, while deep fungi can invade human skin, mucous membranes, deep tissues and internal organs, and even cause systemic disseminated infections. Deep fungal infection in the intestine manifests as fungal enteritis, which can exist independently as infantile candidiasis enteritis, or one of the manifestations of systemic fungal infections, such as AIDS complicated by disseminated histoplasmosis. People who need to be examined: mucosal damage, deep tissue damage, systemic disseminated infection, candida enteritis, disseminated histoplasmosis and other symptoms. Positive results may be diseases: bacterial infection, blastomycosis, onychomycosis, frog fecal mildew considerations 1. Strict aseptic operation to avoid pollution. During the cultivation period, if the growth of contaminated bacteria is found, it should be immediately transferred. 2. On the second day after inoculation, observe daily and record the growth. Positive culture can establish a diagnosis. Negatives need to be cultured for 3 weeks before they can report. 3. Simultaneously culture several tubes or three consecutive cultures, especially specimens in the respiratory tract, intestines, etc., to ensure the reliability of the strains. 4. Powdery colonies of fungi, which should be manipulated in a sterile hood due to spores flying. 5. For the highly infectious bacterial beads, the nozzles are sealed with paraffin when stored. Keeping the infected area clean and dry helps to inhibit the proliferation of fungi and promote skin healing. The infected area should always be washed with soap and water, dried and then sprinkled with talcum powder. Avoid using powders containing corn flour because it promotes fungal growth. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Isolation and culture 【method】 1. Test tube method: Inoculate the collected specimens on the slant surface of glucose protein agar (SDA) medium by aseptic method, inoculate each slope 3 to 4 places, gently cut through, and set 22 ° 25 ° C incubator Continuous culture for 1 to 3 weeks, and make observation records. 2. Small culture: Prepare a sterile glass plate with curved glass rods and slides. Cut the SDA medium into 1cm2 squares with a sterile knife and place them in the center of the slide in the sterile plate to inoculate the strain. Then, take a piece of sterile cover glass on the inoculated square agar base, put a sterile moist cotton ball in the dish, put it into the 22-25 °C incubator, and after the growth, take out the coverslip and repeat On the slide, the cotton lactic acid blue stained microscope was observed. [species identification] According to the morphology, size, structure, edge, color, growth rate, surface properties, sinking phenomenon and microscopic morphology of the strain, when the strain is determined, it should be identified by identification medium and biochemical test. Not suitable for the crowd The test is a non-invasive test with no specific contraindications. Adverse reactions and risks The test is a non-invasive test that does not cause serious complications or other hazards.

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