hookworm culture

The hook-and-loop culture method is an auxiliary test method for detecting parasites. Also known as test tube culture method. Under suitable temperature and humidity conditions, hookworm eggs develop and hatch larvae within a few days. Generally, after 3 to 5 days, they can be observed with naked eyes or magnifying glass. The detection rate is 7 times of the direct smear method, which is also better than Saturated saline flocculation method, hatched filamentous mites can be used for species identification. This phase check can be used to determine the corresponding symptoms. Basic Information Specialist classification: Digestive examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: A negative test result indicates that the subject should be in a normal condition. Positive: A positive reaction indicates that the subject may be infected with hookworms. Tips: The whole process takes more than 5 days. When the temperature is low, keep warm and add water every day. Normal value No parasite eggs or other pathogens were found to be negative during the examination. Clinical significance Abnormal results This method diagnoses schistosomiasis, and found that the edema indicates that there is live schistosomiasis in the patient, which is the most commonly used method for schistosomiasis pathogen detection, and the detection rate is higher than the natural precipitation method. People who need to be examined have abnormal gastrointestinal tracts. Positive results may be diseases: hookworm disease, hookworm duodenitis syndrome, hookworm dermatitis, pulmonary hookworm disease considerations Contraindications before the test: Do not take anti-parasitic drugs before the test, so as not to affect the results. Requirements for inspection: The whole process takes more than 5 days. When the temperature is low, it should be kept warm and water is added every day. Inspection process Take 1cm × 10cm test tube and add about 1ml of cold water. Cut the filter paper into a "T"-shaped paper strip that is as wide as the test tube but shorter than the test tube. The horizontal strip part is written with the pencil and the size of the fecal bean is taken. Apply evenly to the 2/3 part of the strip, insert the strip into the test tube, immerse the lower end in water (be careful not to mix the feces into the water), and incubate at 20 °C ~ 30 °C. During the cultivation process, cold water is dripped along the wall of the pipe with a dropper every day to replenish the water evaporated in the pipe. Do not flush on the fecal membrane when adding water. After 5 days, check the presence or absence of hooks in the bottom of the tube with the naked eye or a magnifying glass. The hookworm is transparent and acts as a serpentine. If it is negative, it should be continued until the 7th day; if the temperature is too low, the culture tube can be placed in warm water (about 30 °C) for several minutes, and then observed. If you need to identify the species, you can take the sediment from the bottom of the culture tube and observe it on the glass slide. When the temperature is low, put the test tube in warm water (about 30 °C) for a few minutes before checking. Not suitable for the crowd The test is a non-invasive test with no specific contraindications. Adverse reactions and risks This test is a non-invasive test that does not cause serious complications or other hazards.

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