Parasitic excrement and secretion examination

Parasite excreta and secretion testing is an auxiliary method for examining parasites. The secretions to be examined are urinary sputum, duodenal juice and bile, urine, hydrocele, and vaginal secretions. Through this examination, the corresponding symptoms can be judged. Do not take anti-parasitic drugs before testing to avoid affecting the results. Basic Information Specialist classification: Digestive examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: not fasting Tips: Do not take anti-parasitic drugs before inspection, so as not to affect the results. Normal value No parasites were found to be negative in excreta and secretions. Clinical significance Abnormal results may be found in urinary sputum sputum sputum eggs, amebic trophozoites in the lysate, scorpion scorpion scorpion larvae, larvae of faecalis, cockroaches, cockroaches, dust mites, etc.; Encapsulation of Pneumocystis can also occur in sputum, but the detection rate is very low. Duodenal juice and bile can be used to check Giardia lamblia trophozoites, Clonorchis sinensis eggs, Fasciola hepatica eggs and B. vannamei eggs; in the bile of patients with acute amoebic liver abscess Found a large trophozoite. Urine can be found in Trichomonas vaginalis, microfilariae, and Schistosoma japonicum egg sheath effusion mainly to check Ban's microfilaria. Vaginal secretions are checked for Trichomonas vaginalis. Patients in need of examination for patients with digestive tract diseases or patients with urinary tract lesions. Positive results may be diseases: Intestinal piriformosis, parasitic diseases, non-parasitic liver cysts Contraindications before the test: Do not take anti-parasitic drugs before the test, so as not to affect the results. Requirements for inspection: Care should be taken when taking secretions and should be observed carefully. Inspection process 1. Sputum check: (1) Paragonimus egg examination Direct smear method: first add 1-2 drops of physiological saline on the clean glass slide, pick a little sputum, preferably sputum with rust color, apply it to the aponeurosis, and cover the microscopy. If the paragonimiasis egg is not found, but there is a Charcot-Ryden crystal, suggesting that it may be a paragonimiasi patient. If the smear test is negative, you can use the concentration method instead. Concentration method: collect the sputum for 24 hours, place it in a glass cup, add an equal amount of 10% NaOH solution, stir well with a glass rod, and put it into a 37 ° C incubator. After several hours, the mash is digested into a dilute liquid. Dispense in several centrifuge tubes, centrifuge at 1500 rpm / min for 5-10 minutes, discard the supernatant, and take a few drops of smear for inspection. (2) Amoeba trophozoite examination in the dissolved tissue: Take fresh sputum for smear. When it is cold, pay attention to the glass slide insulation on the stage. High magnification observation, such as the amoeba trophozoites, can be seen to extend the pseudopod and make directional movement. (3) Other worm larvae and mites mentioned above should be examined by concentration method. 2. Duodenal juice and bile examination method: Each part of the duodenal drainage can be dripped onto the glass slide, and the microscopic examination is performed after the cover piece is added. In order to improve the detection rate, each part of the drainage liquid is diluted and stirred with physiological saline, and then dispensed into a centrifuge tube, centrifuged at 2000 rpm/min for 5-10 minutes, and the sediment smear microscopy is taken. If the drainage fluid is too viscous, it should be digested with 10% NaOH and then centrifuged. The Giardia trophozoites in the drainage are often attached to small pieces of mucus, or the worms are aggregated into flakes. The eggs of the flukes of the liver and the eggs of the ginger are not easily identified, but the former can appear in the bile; the latter is only found in the duodenal juice. 3. Urine: Take 3 to 5 ml of urine, centrifuge (2000 rpm / min) for 3 to 5 minutes, and then take sediment microscopic examination. 4. Hydrocele: After the scrotal skin is disinfected by iodine alcohol, the hydrocele is taken with a syringe for direct smear examination. It can also be diluted with physiological saline to obtain a sediment microscopy. 5. Vaginal secretions: Direct smear method: use a sterile cotton swab to take secretions from the vaginal sputum, cervix and vaginal wall of the subject, and then use the saline smear microscopy to find the active worm. When the weather is cold, you should pay attention to heat preservation. Hanging drop method: first apply a thin layer of Vaseline on the periphery of a coverslip, and 1-2 drops of normal saline in the middle. The vaginal secretions were applied to physiological saline, and the flaps were flipped over carefully on a glass slide with a concave hole. The two pieces were adhered with a slight pressure, and the droplets were suspended under the cover sheets for microscopic examination. Not suitable for the crowd Patients who have taken anti-parasitic drugs and other drugs may not be affected by the recent history of drug administration. Adverse reactions and risks This test is a non-invasive test that does not cause serious complications or other hazards.

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