Modified Kato Method
The modified Kato method is an auxiliary examination method for examining parasites. The principle is to use fecal quantitative or qualitative thick smear to increase the number of eggs in the field of vision, and can be used for quantitative examination of eggs. Treated with glycerin and malachite green, the fecal membrane is transparent, so that the feces and the eggs are in sharp contrast, which is convenient for light transmission and microscopic examination. Malachite Green softens the field of view to reduce eye strain. Suitable for checking various worm eggs. The method is simple and the eggs will not be lost during the operation. The effect is better. This method should not be used for hard and too thin feces. Foamy feces can form many tiny bubbles under the cellophane, which prevents microscopic examination. It can also be used for quantitative inspection of eggs. This phase check can be used to determine the corresponding symptoms. Basic Information Specialist classification: Digestive examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: The result of the negative test indicates that the body should be in a normal state. Positive: A positive test result indicates that the body has a parasitic infection. Tips: Before the test, the taboo is not to take anti-parasitic drugs before the test, so as not to affect the results. Normal value No parasite eggs or other pathogens were found to be negative during the examination. Clinical significance Abnormal results: Check for parasite eggs or other pathogens. People who need to be examined: those with abnormal digestive tract. Positive results may be diseases: pediatric schistosomiasis, tsutsugamushi precautions Contraindications before the test: Do not take anti-parasitic drugs before the test, so as not to affect the results. Requirements for inspection: 1 The fecal membrane should be spread evenly, not too thick; 2 The transparent time should be moderate (especially for hookworm eggs, the transparent time should be short, not more than 30 minutes). If the fecal membrane is too thick and the transparent time is short, the eggs are difficult to find. If the transparent time is too long, the eggs are deformed and are not easily identifiable. Or because the eggs are also transparent, it is easy to miss the inspection. 3 In quantitative applications, in order to increase the detection rate, three Kato tablets are required for each sample. Inspection process 1 Cover the screen with the manure specimen and scrape the feces that have overflowed from the sieve hole. 2 Snap the dosing plate on the slide, fill the die hole with the manure obtained on the scraper, scrape off the excess, pick up the dosing plate, cover the fecal sample with a glass paper strip containing malachite green glycerin, and flatten it. The platen is pressurized and the fecal sample is laid elliptical between the cellophane and the slide. 3 In the 30 ° C ~ 37 ° C incubator transparent 0.5 ~ 1h, microscopic examination and egg count. 4 The number of eggs per gram of fecal matter (EPG) is the total number of eggs per plate multiplied by 24, multiplied by the coefficient of fecal traits. Not suitable for the crowd Patients who have taken anti-aphid drugs and other drugs may not be affected by the recent history of drug administration. Adverse reactions and risks This test is a non-invasive test that does not cause serious complications or other hazards.
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