Gelatin Liquefaction Test

The gelatin liquefaction test is a test method in which some bacteria can produce an extracellular enzyme-gelatinase, which can decompose gelatin into amino acids, thereby losing coagulability and semi-solid gelatin medium becoming a flowing liquid. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: not fasting Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance The identification of Enterobacteriaceae bacteria, such as Serratia, Proteus, Proteus mirabilis, genus bacillus, etc., liquefiable gelatin, while other bacteria rarely liquefy gelatin. Some anaerobic bacteria such as Clostridium perfringens, Bacteroides fragilis, etc. can also liquefy gelatin. In addition, most Pseudomonas can also liquefy gelatin. Abnormal result Enterobacter can cause gastroenteritis, typhoid fever, bacteremia or sepsis in clinical practice. Among them, intestinal fever is a legal infectious disease. Clostridium, Clostridium, Clostridium perfringens . Pseudomonas is divided into Pseudomonas aeruginosa infection, B. sinensis infection, and snoring. The person in need of examination has the above symptoms. Positive results may be diseases: acute gastroenteritis, tetanus, gas gangrene, Pseudomonas aeruginosa corneal ulcer precautions Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Medium: Gelatin medium. Method 1: Gelatin agar plate method can be divided into four zones after adding 1% gelatin pouring plate to common agar. Each zone is inoculated with a test bacteria, and after 24 to 48 hours, it is cultured on the surface of the medium. A mercury chloride solution (12 g of mercury chloride, 80 ml of distilled water, and 16 ml of concentrated hydrochloric acid) was injected, and if the bacteria liquefy gelatin, a transparent band appeared around the colony. Method 2: The test bacteria were inoculated into gelatin medium, and cultured at 20 ° C for 7 days, and the phenomenon of gelatin liquefaction was observed day by day. If the medium is dissolved at room temperature, it can be placed in the refrigerator for 30 minutes, and then the observation result is taken out, and it is not positive when it is not solidified. Not suitable for the crowd No. Adverse reactions and risks no.

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