Plasma protein C activity assay

The plasma protein C activity measurement (pc) is a measurement of the C activity of blood proteins in plasma, and the plasma protein C activity measurement test process: plasma proteins are separated by blood sampling, and plasma protein C activity is measured. A protein C activator (extracted from snake venom) was added to the plasma to be tested. PC is activated as activated protein C (APC), APC acts on the chromogenic substrate ChromozymPCA, releasing the chromogenic group p-nitroaniline (PNA), and the color depth is parallel to the PC content in the plasma. The AT is calculated from the standard curve. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Pay attention to rest before the examination, and take blood on an empty stomach. Normal value 102.5% ± 20.1%. Clinical significance Abnormal result Reduced C activity Common in congenital PC defects, according to C activity determination of A and PC, antibodies can be divided into type I (both PCAg and PCA reduced) and type II (normal PCAg and decreased PCA). It is also possible to have acquired PC defects such as DIC, liver dysfunction, postoperative, oral dicoumarin anticoagulant, respiratory distress syndrome and the like. The people who need to be examined have weak, weak, sleepy, pale skin, mucous membranes, palpitations, dizziness, headache, tinnitus, vertigo, inattention, and lethargy. Low results may be diseases: diffuse intravascular coagulation, fulminant liver failure precautions Inappropriate crowd: pregnant women, newborns. Taboo before the test: pay attention to rest, blood on an empty stomach. Requirements for inspection: No special requirements. Inspection process Plasma proteins were isolated by blood draw and plasma protein C activity was measured. Protein C activator (extracted from snake venom) was added to the tested plasma, PC was activated to activated protein C (APC), and APC was applied to the chromogenic substrate ChromozymPCA to release the chromogenic group p-nitroaniline (PNA). The depth of color development is in parallel with the amount of PC in the plasma being examined. The AT is calculated from the standard curve. Not suitable for the crowd 1. Patients taking drugs such as oxidative drugs and steroid hormones may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. 3. Pregnant women and newborns are not suitable for this examination. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.

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