sperm immobilization test

Sperm agglutination antibodies or brake antibody sperm in serum or seminal plasma can be detected by a sperm braking test. Braking test is best to take blood in the morning on an empty stomach. Coffee, tea, high-sugar and cola drinks should be fasted. Drinking should be avoided in the dinner one day in advance, and high-fat, high-protein diet should be prohibited. In addition, it should be before blood draw. Pay attention to maintaining a normal diet within 3 days. Basic Information Specialist classification: male examination inspection classification: blood examination Applicable gender: whether the male is fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Generally normal. Positive: A positive indication may be infertile. Tips: Avoid drinking alcohol for dinner one day in advance, and prohibit high-fat, high-protein diet. Normal value No sperm agglutinating antibody or brake antibody is present and negative. Clinical significance Abnormal result The presence of sperm agglutinating antibodies or brake antibodies after testing indicates infertility. People who need to be checked Female infertility patients. Positive results may be diseases: infertility, serum anti-sperm antibody positive infertility precautions Taboo before inspection: (1) Controlling diet 1. Most inspection items require blood sampling on an empty stomach in the morning, and coffee, tea, high sugar and cola beverages should be fasted; 2, one day in advance of dinner should avoid drinking, prohibiting high-fat, high-protein diet; 3, should pay attention to maintain a normal diet within 3 days before the blood draw. (B) to avoid drug effects The effects of drugs on biochemical tests and bacterial culture are complex. Therefore, if possible, the subject should stop taking all medications 1-2 days before the test. If medication is necessary for treatment, the effects of the drug should be considered when analyzing the results of the test. (3) Avoiding the impact of exercise 1, strenuous exercise can make many blood components change, even for more than 24 hours, so after vigorous exercise should not immediately take blood and walk quickly, at least 10-15 minutes after rest, then take blood; 2, should be in a calm state to draw blood, to avoid emotional excitement. Requirements for inspection: 1. In order to ensure that you can accurately understand your physical examination results after the medical examination, please fill in the medical examination form and each test sheet carefully before the medical examination. The writing should be clear, especially the name. 2, after taking serum, you should sit still for 15-20 minutes. Inspection process I. Serum preparation: Since the guinea pig serum contains substances that are toxic to human sperm, the guinea pig serum used as a complement should be checked one by one before the combination, and only the guinea pig serum without sperm braking effect is combined with the hemolysis reaction. Serum complement titer (C'H) was measured, and the unprotected female serum without sperm immobilization was adjusted to a complement titer of about 200C'H to ensure that the brake response system had sufficient complement (10C'H) to participate. The guinea pig serum was dispensed in a small test tube at 0.5 mL, and stored at -70 ° C until use. 1. Preparation of guinea pig serum for complement: 10 to 15 guinea pigs were used at one time, blood was collected from the heart, serum was separated at low temperature, and the sperm was tested for toxicity by human sperm test. The specific method is as follows. test group: Virgin serum: 0.25mL; Sperm suspension: (40 × 106 / mL) 0.025mL} 32 ° C / 60 min sperm activity rate (T%); Guinea pig serum to be tested: 0.05 mL. Control group: Virgin serum: 0.25mL; Sperm suspension: (40 × 106 / mL) 0.025mL} 32 ° C / 60 min sperm activity rate (C%); Inactivate guinea pig serum (or no guinea pig serum): 0.05 mL. Comparison of the two: C / T ≥ 1.2 was judged to be toxic, < 1.2 was considered non-toxic. After guinea pig serum of 1.2 or less was combined, the complement titer was measured. In addition, human serum of AB blood type is used as complement, but the complement titer of AB blood type human serum is much lower than that of guinea pig serum. The addition of 0.05 mL serum to the reaction system still does not fully detect the brake antibody, so it is generally not used. Second, sperm washing Semen can be achieved by husband or others and can achieve the same result. However, since most of the sperm-braking antibodies in the blood of infertile women are antibodies produced by seminal plasma antigens, seminal plasma has the effect of neutralizing antibodies, which may affect the results of the assay. Therefore, when preparing a sperm suspension, the sperm should first be washed with 10% rabbit inactivated serum, and the seminal plasma component should be removed as much as possible to avoid reducing the titer of the sperm immobilized antibody. Not suitable for the crowd 1. Patients who have taken thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.

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