indirect antiglobulin test

The indirect anti-human globulin test is to check whether there is free incomplete antibody in serum, which is an important basis for the diagnosis of autoimmune hemolytic anemia (AIHA). AIHA can be divided into three types by specific monovalent antiserum: IgG/C3 positive, accounting for 67%; IgG alone, accounting for 20%; C3 alone, accounting for 13%; autoimmune anemia, (IgG) type Hemolytic anemia, the direct response of this test is often strongly positive, the indirect reaction is mostly negative, but can also be positive. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: The body is in a normal state. Positive: May have autoimmune anemia, IgG type caused by hemolytic anemia and other diseases. Tips: Check the fasting before the blood, it is better to take blood in the morning. Normal value The indirect anti-human globulin test was negative. Clinical significance Abnormal result There is a corresponding incomplete antibody in the test serum in the test positive body. 1, autoimmune anemia, hemolytic anemia caused by IgG type, the direct response of this test is often strongly positive. 2, drug-induced immune hemolytic anemia, and indirect reactions to co-react disease. 3. The cold agglutinin syndrome is directly positive. 4, neonatal allogeneic hemolytic disease, due to Rh blood group incompatibility caused by hemolytic disease, direct strong positive, lasting for several weeks, transfusion blood transfusion within a few days can be negative, due to "ABO" blood group incompatibility caused by hemolytic disease, the results often Negative or weakly positive. 5. The transfusion reaction caused by the incompatibility of red blood cell type, ABO or Rh blood type is not transfusion, the donor's red blood cells are sensitized by the recipient's blood group antibody, and the direct reaction is positive before the donor is completely destroyed by the sensitized red blood cells. 6, other in infectious mononucleosis, SLE, malignant lymphoma, chronic lymphocytic leukemia, cancer, lead poisoning, nodular periarteritis, Evan's syndrome, etc., the patient's direct response can also be positive, array In patients with chilly hemoglobinuria, the direct response to anti-complement serum after an acute attack is often positive. People who need to be checked There are people who are weak and weak, sleepy, pale skin, mucous membranes, palpitations, dizziness, headache, tinnitus, vertigo, inattention, and lethargy. Positive results may be diseases: autoimmune hemolytic anemia, Rh blood group incompatibility, chronic lymphocytic leukemia considerations Before the examination: fasting, taking blood in the morning is better When checking: Relax your body and actively cooperate with your doctor. Inspection process Collect venous blood of the test subject, put it into a test tube containing rabbit brain powder or other coagulant, centrifuge for 10 minutes, take the supernatant, or venous blood into the test tube without anticoagulant, and leave it at 4 ° C for 12 hours, or After standing at 37 ° C for 2 hours, centrifuge, 2000 r / min, 10 minutes, take the supernatant, the serum of the test subject was added to the small hole of the test card, the negative control serum was added to another small hole, and after centrifugation, the comparison was judged as Positive or negative. Not suitable for the crowd 1. Patients who have taken thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.

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