Plasma Factor XIII Deficiency Screening Test

The plasma factor XIII deficiency screening assay is a qualitative analysis of plasma factor XIII in humans for the diagnosis of congenital and acquired factor XIII deficiency. The test result was not positive, that is, the XIII factor was lacking. Factor XIII reduction is seen in congenital and acquired factor XIII deficiency. Acquired factor XIII deficiency is much more common than congenital factor XIII deficiency. Acquired XIII factor deficiency is seen in the third to sixth day after surgery, various severe liver diseases, mercury, lead, phosphorus poisoning. Leukemia, lymphoma, multiple myeloma, lupus erythematosus. Rheumatoid arthritis, uremia and diffuse intravascular coagulation. Increased factor XIII is seen in premature infants, women with bleeding during pregnancy, dyspnea syndrome and hyaline membrane disease, myocardial infarction with decreased fibrinolytic activity and increased heparin tolerance. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: After 8 pm on the day before the medical examination, you should fast, so as not to affect the detection of indicators such as blood glucose in the second sky. Normal value Normal people do not dissolve in the blood clot at 24 hours. Clinical significance Abnormal result The test result was not positive, that is, the XIII factor was lacking. Factor XIII reduction is seen in congenital and acquired factor XIII deficiency. Acquired factor XIII deficiency is much more common than congenital factor XIII deficiency. Acquired XIII factor deficiency is seen in the third to sixth day after surgery, various severe liver diseases, mercury, lead, phosphorus poisoning. Leukemia, lymphoma, multiple myeloma, lupus erythematosus. Rheumatoid arthritis, uremia and diffuse intravascular coagulation. Increased factor XIII is seen in premature infants, women with bleeding during pregnancy, dyspnea syndrome and hyaline membrane disease, myocardial infarction with decreased fibrinolytic activity and increased heparin tolerance. Premature infants, pregnant women, patients with liver disease and patients with blood diseases need to be examined. Positive results may be diseases: pyrimidine 5'-nucleotide deficiency precautions Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. A blood sample was taken to separate the plasma. The test plasma, white clay - cephalin suspension 0.1 ml each, mix, 37 ° C water bath for 3 min, gently shake several times. Add 0.1ml of 0.025mol/L calcium chloride solution, start the stopwatch immediately, shake constantly and observe the time of fibrin wire. Repeat 2 times, take the average and make a normal control. To the test tube, 109 ml/L sodium citrate or 0.1 mol/L sodium oxalate solution 0.2 ml was added, and then 1.8 ml of the test blood was added and mixed, and the plasma was separated by low speed centrifugation. Take 1 small test tube, add 0.1ml of plasma and rabbit brain powder leaching solution, pre-warm at 37 °C, then add 0.1ml CaCl2 solution to pre-warm at 37 °C, immediately start the stopwatch, continuously tilt the test tube, until the liquid flow stops, the time is Prothrombin time. Repeated operation 2 to 3 times, averaged, and served as a normal control. Not suitable for the crowd A patient with hemophilia and severe clotting factor deficiency. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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