Acidified glycerol hemolysis test

The acidified glycerol hemolysis test is a time to check when the red blood cells are present in the hypotonic solution sodium chloride phosphate buffer, and the absorbance of the red blood cell suspension is reduced to 50% (AGLT50). That is, AGLT50 is less than 290s, which is common in hereditary spherocytosis (25-150s), renal failure, chronic leukemia, autoimmune solution, and pregnant women. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Pay attention to rest and keep blood on an empty stomach. Normal value Normal person AGLT50>290s. Clinical significance Abnormal result The result of acidified glycerol hemolysis test is positive, that is, AGLT50 is less than 290s, which is common in hereditary spherocytosis (25-150s), renal failure, chronic leukemia, autoimmune solution and pregnant women. People who need to be checked Pregnant women, people with abnormal kidney function. Precautions Taboo before the test: pay attention to rest, keep blood on an empty stomach. Do not wear clothes that are too small or too tight in cuffs to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood draw, causing blood vessels in the arms. Avoid strenuous exercise. Requirements for inspection: The inspection may take a long time and you need to wait patiently for the result. Inspection process The peripheral blood mainly has ear lobe blood and fingertips to take blood. The baby can take blood from the heel. The earlobe has a lighter blood pressure, but the blood volume is less, especially those with smaller earlobes are more difficult to take blood. The blood pressure of the fingertips is more obvious, but the blood collection is more, especially for the blood routine test, the stable measurement results can be obtained. The skin should be cleaned before blood collection. Do not take blood immediately after entering the room in the cold outdoor winter. After the body is warm, especially the ear drops and hands should be warmed up. Do not use hot water to heat your hands before taking blood. Keep your fingers dry. If your fingertips have wounds, paronychia, redness or skin disease, avoid using this finger. Fingertip blood collection generally uses the ring finger, because the ring finger will not affect the daily function of the hand after piercing, of course, the middle finger or index finger can also be used, no special difference. When collecting blood in the ear, remove the earrings and other hanging ornaments on the earlobe. Do not hang up immediately after blood collection. After blood collection, apply sterile cotton blocks or other disinfecting hemostatic articles to pinch the needle and puncture the area. Do not touch the dirt. Do not wash your hands immediately. Not suitable for the crowd A patient with hemophilia and severe clotting factor deficiency. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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