Neutrophil count

The neutrophils in the peripheral blood of normal people are mostly lobulated with 3 leaves, less rod-shaped nucleated cells (0.01-0.05), and the normal ratio between rod-shaped nucleus and lobulated nucleus is 1:13. If the ratio increases (ie, rod-shaped neutrophils), or the more naive stage of the granulocytes before the rod-shaped nucleus appears, the nuclear left shift. When the ratio is decreased (indicating that the lobular granulocyte is increased) or the granulosa with more than 5 leaves in the lobes exceeds 0.03, it is called nuclear right shift. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: After taking blood, you need to press it at the pinhole for 3-5 minutes to stop bleeding. Normal value Blood cell automatic counter (0.04 ~ 0.5) × 109 / L (40 ~ 500 / mm3). Clinical significance (1) Neutrophil enlargement 1 acute infection A. Local infections, carbuncles, etc. B. Systemic infection with pneumonia, sepsis, suppurative tonsillitis, purulent meningitis, epidemic cerebrospinal meningitis, rheumatic fever, acute appendicitis, diphtheria, acute cholecystitis, etc. 2 poisoning A. Drug-induced lead, mercury, sleeping pills, digitalis, antipyrine, arsenic, etc.; insect and heterologous protein absorption. B. Metabolic uremia, diabetic ketoacidosis, gout, pregnancy poisoning, etc. 3 acute hemorrhage of blood diseases, acute hemolysis, acute and chronic granulocyte leukemia, Hodgkin's disease, polycythemia vera, etc. 4 tissue necrotic myocardial infarction, gangrene, neonatal necrosis, burns, etc. 5 other cerebral hemorrhage, brain tumor, postoperative surgery, Cushing's syndrome, mucinous edema, malignant tumor, cachexia and so on. (2) neutropenia 1 severe infection with severe sepsis, miliary tuberculosis, etc. 2 special infections with typhoid fever, paratyphoid fever, viral infectious diseases, rickettsial infection, protozoal disease (plassum, kala-azar, protozoal infection). 3 blood disease aplastic anemia, pernicious anemia, leukopenic leukemia, acute and chronic lymphocytic leukemia, malignant lymphoma, multiple myeloma, paroxysmal nocturnal hemoglobinuria. 4 drugs organic lysozyme, anti-tumor drugs, anti-thyroid drugs, anti-caries drugs, sulfonamides, phenothiazines, chloramphenicol, oxytetracycline and so on. 5 Radiation radiation sickness, etc. 6 collagen disease systemic lupus erythematosus and the like. 7 spleen disease Banti syndrome, cirrhosis, Felty syndrome and the like. 8 congenital diseases, periodic neutropenia, congenital neutropenia, familial benign eosinophilia. 9 other cachexia, hemodialysis, etc. Low results may be diseases: high pneumonia results may be diseases: leukemia considerations Neutrophilic granulocytes were significantly elevated, lymphocytes were significantly reduced, and eosinophils disappeared, indicating that the infection was extremely serious, the condition was dangerous, and the prognosis was extremely poor. Inspection process (1) Take a small drop of blood on one end of the slide, and use a push piece to push the circumference around 35 ° ~ 45 ° to leave a proper amount of voids, which can distinguish the thin blood of the head, body and tail. The length of the blood film is not less than 2.5 cm, and the remaining space to the other end of the slide is about 1 cm. The blood film is dried and stained. (2) Wright's Giemsa composite staining method: flat blood sample on the staining rack, add 3 to 5 drops of staining solution, immediately cover the blood film, add about 5 to 10 drops of buffer after about 30s, gently shake the glass The tablets or lightly blow the mixture to mix the dye solution with the buffer solution. After 5 to 10 minutes, the dye solution is washed away with water and dried for microscopic examination. (3) Rapid method: place the rapid dyeing liquid A and liquid B in the appropriate size dyeing tank, immerse the blood film in the liquid for 30s, wash it, then immerse it in the liquid for 30s, wash it, and dry it for microscopic examination. (4) Microscopic examination: Select the junction of the meninges and tails, and the red blood cells have not overlapped with oil mirrors. The examination should have a certain direction from top to bottom and left and right, and take into account the edges of both sides of the long film of the blood film, otherwise it will affect various cells. Detection rate. Count 100 to 200 white blood cells, classify them according to their morphology, and find the percentage. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Subcutaneous hemorrhage: subcutaneous hemorrhage due to less than 5 minutes of compression time or blood draw technique.

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