Glucose 6-phosphate dehydrogenase fluorescent spot test

Glucose 6-phosphate dehydrogenase fluorescence spot assay is a fluorescence method for the determination of glucose 6-phosphate dehydrogenase, in the presence of glucose 6-phosphate dehydrogenase and NADP+, glucose 6-phosphate dehydrogenase can reduce NADP+ to NADPH The latter fluoresces under ultraviolet light. The absorption peak of NADPH is at a wavelength of 340 nm, and the amount of NADPH can be generated per unit time to determine glucose 6-phosphate dehydrogenase activity. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Before the inspection, the beans and their products are forbidden, and the oxidative drugs are stopped. Normal value The test results have no fluorescent spots. Clinical significance Abnormal result The result of the examination was a fluorescent spot, and the activity of glucose-6-phosphate dehydrogenase was lowered. The clinical appearance of anemia, skin, sclera yellow staining, mild swelling of the liver and spleen or normal. May have hemolytic anemia. Fluorescence points may also occur in patients with drug reactions (such as primaquine, sulfapyridine, acetanilide, etc.), faba bean disease, and infection. The people who need to be examined have dizziness, headache, palpitations, difficulty breathing, abdominal pain, and low back pain. People with severe hemoglobinuria can cause symptoms of kidney failure. Precautions Taboo before the inspection: banned the broad beans and their products, stop taking oxidative drugs. Requirements for examination: Do not tamper with venous blood collection. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected; but it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Not suitable for the crowd Patients with hemoglobin <60 g/L. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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