Coagulation factor activity assay

Coagulation factor activity is a measure of the activity of various coagulation factors in the human body. Coagulation factors play a very important role in blood coagulation. Measuring the activity of various coagulation factors helps to determine the type of hemophilia, blood. The severity of the disease and the clotting status under certain pathological conditions. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Reduced in hemophilia. Normal value: Factor VIII: 78-128 Factor IX: 68-128 Above normal: Increased in hypercoagulable state and thrombotic disease. negative: Positive: Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value Factor VIII: C78% to 128%; Factor IX: C68% to 128%. Clinical significance reduce 1, VIIIC reduction is seen in hemophilia A, vascular hemophilia, disseminated intravascular coagulation. 2, factor IXC reduced in hemophilia C, liver disease, vitamin K deficiency, disseminated intravascular coagulation, oral anticoagulants. 3, factor XIC reduction can be seen in congenital factor XI deficiency, vitamin K deficiency, diffuse intravascular coagulation. 4, factor XIIC reduction can be seen in congenital factor XII deficiency, disseminated intravascular coagulation, liver disease and so on. 5, factor IIC, factor VC, factor VIIC, factor XC decreased, seen in congenital coagulation factor deficiency or acquired coagulation factor reduction, such as liver disease, vitamin K deficiency, disseminated intravascular coagulation, oral anticoagulant and blood Anticoagulant substances, etc. Raise Found in hypercoagulable state and thrombotic diseases, such as deep vein thrombosis, pulmonary embolism, nephrotic syndrome, pregnancy-induced hypertension syndrome, malignant tumors. Factor VIII is also seen in liver disease. Low results may be diseases: high hemophilia results may be diseases: precautions for pulmonary embolism Taboo before the test: Do not eat too greasy, high-protein foods the day before the test, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. After 8 pm on the day before the medical examination, you should fast. It is forbidden to take aspirin. It is forbidden to take various drugs before the test to avoid any impact. Requirements for examination: When taking blood, you should relax your mind to avoid the contraction of blood vessels caused by fear and increase the difficulty of blood collection. Inspection process Take the standard and prepare the standard solution with the effective price (U/mL) of 5, 6, 7, 8, 9, and 10 with physiological saline. Take another 6×10cm plastic test tube, add 450μl of 0.1% fibrinogen solution, preheat for 5min in 37°C water bath, and then take 50μl of each of the above six concentrations of standard solution preheated at 37°C. Add to each of the above test tubes, immediately time and shake well, and record the coagulation time. Each concentration was measured 2 times, averaged, and a standard curve was made. The sample to be tested is diluted with physiological saline to a suitable concentration, 50 μl is taken, and measured in parallel according to the measurement method of the standard curve twice, averaged, and the titer of the sample is calculated according to a standard curve or a regression equation. Not suitable for the crowd People who take aspirin for a long time. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.

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