antiglobulin test

The anti-human globulin test is a common method for examining incomplete antibodies and is the most important test for the diagnosis of autoimmune hemolytic anemia. Generally divided into direct test and indirect test. The purpose of the direct test is to examine incomplete antibodies on the surface of red blood cells. The purpose of the indirect test is to check for the presence of free incomplete antibodies in the serum. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Can be seen in neonatal alloimmunopathies. Tips: Actively cooperate with the doctor during the examination. Normal value The direct test was negative. Indirect test was negative. Clinical significance (1) Autoimmune hemolytic anemia warm antibody type direct method positive, indirect method negative. (2) Drug-induced immune hemolytic anemia The 1α-methyldopa type was positive both directly and indirectly. 2 penicillin type positive method positive, indirect method negative. 3 Fu Aiting type positive method positive. (3) The cold agglutinin syndrome is positive by direct method and negative by indirect method. (4) Newborn allogeneic hemolytic disease is positive both directly and indirectly. (5) Direct method positive infectious mononucleosis, systemic lupus erythematosus, malignant lymphoma, chronic lymphocytic leukemia, cancer, lead poisoning, nodular polyarteritis, Evans' syndrome (Evans Syndrome, idiopathic thrombocytopenic purpura with acquired hemolytic anemia, paroxysmal cold hemoglobinuria, etc. (6) Direct method negative hereditary spherocytosis. Positive results may be diseases: chronic lymphocytic leukemia in the elderly, hemolytic anemia in children, brucellosis considerations This test is more specific and less sensitive to the diagnosis of immune hemolysis, and is prone to false negative results. False negatives may occur in the following cases: the amount of IgG on the surface of the patient's red blood cells is small, the titer of the reagent is not high, the affinity of the autoantibody is low, and a large amount of adrenal hormone is cited in the early stage of the disease. Inspection process Anti-human globulin test (Coombs test) including direct anti-human globulin test and anti-human globulin indirect test Direct anti-human globulin test The anti-human globulin direct test is used for allogeneic immunity caused by neonatal hemolytic disease, autoimmune hemolytic anemia, drug-induced hemolysis, and blood transfusion. Anti-human globulin indirect test The anti-human globulin indirect test is mainly used to detect the reaction of cells and sensitized antibodies in vitro. It can be used in human antibody test, blood group identification and compatibility test and special research, such as anti-human globulin consumption test, mixed agglutination reaction, Leukocyte and platelet antibody test tests, etc. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Generally not.

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