cross-matching test

The cross-matching test refers to the red blood cell suspension of the blood donor's serum plus blood donor, the blood donor's serum plus the recipient's red blood cell suspension, and an agglutination test. The former is called the main test and the latter is called the sub-test. The purpose is to verify whether the donor and recipient ABO blood group identification is correct and to prevent hemolytic transfusion reactions. In addition, irregular lectins of the ABO blood group system and other blood group antibodies other than the ABO system can be detected. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Fasting for 12 hours before taking blood, taking fresh blood for inspection. Normal value No agglutination (also hemolysis) in both the primary test and the secondary test indicates that the blood donor and the blood donor ABO blood type match. Clinical significance The main function is based on the blood type identification. It is further confirmed by the cross-matching test that there is no blood-type antigen-antibody antibody reaction between the recipient and the blood donor to ensure the blood transfusion safety of the recipient. People who need to be tested need blood transfusions. Precautions Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. Fasting for 12 hours before taking blood, taking fresh blood for inspection. When checking: When you take blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process Colloidal medium matching method 1. First prepare the blood samples of the recipients and blood donors. 2. A 2% red blood cell suspension prepared with the serum of the recipient; a 2% red blood cell suspension prepared with blood donor serum. 3, the main side plus blood donor serum 0.5ml, blood donor serum prepared 2% red blood cell suspension 0.25ml. 4, the secondary side plus blood donor serum 0.5ml, 2% red blood cell suspension 0.25ml. 5. Incubate for 1 hour in a 37-degree water bath, and observe whether there is agglutination or hemolysis. Microcolumn gel test Microtubes GelTest (MGT) is a new method used in domestic laboratories and used for cross-matching in the 1990s. In some advanced countries, it has become a routine erythrocyte blood group serological detection technique. The use of condensed amines for the determination and identification of incomplete antibodies and serological tests such as cross-matching have also been rapidly promoted in China. The method is simple, short in time and obvious in results. Not suitable for the crowd There are people with blood infections. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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