Antithrombin III activity and antithrombin III antigen

Antagonism with the function of the coagulation system in the human body is the anticoagulant system, which maintains a dynamic balance under normal conditions, thereby maintaining the normal flow of blood in the blood vessels. Antithrombin III is the most important component of the anticoagulant system. It is synthesized by the liver and is a multifunctional serine protease inhibitor that inhibits thrombin generation, has strong anticoagulant activity, and accounts for plasma anticoagulant activity. 70%. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Below normal, it is easy to cause thrombosis. Normal value: Antithrombin III activity: 0.90-1.20L Antithrombin III antigen: 250-330L Above normal: Hemorrhagic period such as hemophilia, leukemia and aplastic anemia, simple purpura, nephropathy, uremia, pregnancy-induced hypertension syndrome, and the like. negative: Positive: Tips: Actively cooperate with the doctor during the examination. Normal value Antithrombin III activity is 0.90 to 1.20 (luminescent substrate method). Antithrombin III antigen 250 ~ 330mg / L (immunotactic turbidimetry). Clinical significance Reduced in: Congenital and acquired antithrombin deficiency, such as liver disease, disseminated intravascular coagulation, postoperative surgery, myocardial infarction, angina pectoris, cerebrovascular disease, glomerular disease, venous thrombosis, etc. Raised in: Hemorrhagic period such as hemophilia, leukemia and aplastic anemia, simple purpura, nephropathy, uremia, pregnancy-induced hypertension syndrome, and the like. Low results may be diseases: Hereditary antithrombin III deficiency results are high. Possible diseases: Leukemia considerations The use of progesterone, estrogen contraceptives, anticoagulants can increase the results. Therefore, these drugs must be banned before testing. Inspection process (1) The dissolved rabbit anti-human AT-III serum (Reagent A) was labeled as half of the dilution factor by bottle label, and diluted with an electrophoresis buffer for use. Further, water was used to prepare a 20 g/L agarose solution. Mix the above two solutions at 56 ° C, and pour into two glass plates with 80 mm × 80 mm × 1.5 mm "U" shaped mold frame. After solidification, make a row of loading on one side of the plate. Hole, hole diameter 2mm, hole pitch 3mm. (2) The dissolved standard plasma (Reagent B) was diluted with water 1:2, 1:4, 1:8, 1:16, and the sample was also diluted 1:5 with water, and then quantified by a micropipette. Add the sample well, 5 μl per well, and electrophoresis for 6 h at a potential gradient of 15 V/cm (the sample well should be placed on the negative side). (3) After electrophoresis, the gel plate was placed in a 4.4 mmol/L phosphomolybdic acid solution, fixed for 15 min, and the height of each rocket peak from the center of the sample to the peak tip (mm) was measured. The peak height (X) of the plasma at different dilution standards was used to prepare a standard curve on the ordinate paper for the corresponding AT-III antigen content (Y). Then, the peak height of the sample is directly read out of the AT-III antigen content on the standard curve; or the linear regression operation is performed, and the regression formula Y=bx+a is obtained, and the peak height of the sample is substituted into the formula x to obtain the ATII: The content of Ag is Y. Not suitable for the crowd Generally no taboos. Adverse reactions and risks Concurrent infection.

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