Leptospira latex agglutination test

Leptospira is a kind of slender soft spiral, which is widely distributed in many wild animals and domestic animals. It can cause systemic infections in humans and mammals. The carrier animal is the main source of infection of Leptospira, which is mainly contact and transmission. Rats and cats are the main source of infection in China. When a person is infected, collecting serum IgM antibodies for 2 weeks of onset is of great value for early diagnosis of leptospirosis. After 2 to 4 weeks, IgG antibodies appeared, followed by IgG antibodies. Basic Information Specialist Category: Infectious Diseases Inspection Category: Microscopy Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal when negative. Positive: A positive titer higher than 1:40 can be used as a diagnostic basis for leptospirosis. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Normal value 0 to 1:40. Clinical significance A positive titer higher than 1:40 can be used as a diagnostic basis for leptospirosis. Positive results may be diseases: predator trematode migration considerations Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process Inspection method: draw blood. (1) The black-painted glass plate on the back side is cleaned and dried on the front side, and cut into small circles (about 2 cm in inner diameter) with red paint, or cut into squares. The patient's serum was inactivated at 56 ° C for 30 min. (2) Simultaneously perform the milk coagulation inhibition test and the milk coagulation test: mark the left and right sides of the glass plate, add two drops of the patient's serum, add 1 drop of the immune serum on the left side, and mix at room temperature for 1-2 minutes. Add 1 drop of latex antigen solution on both sides, mix well, and observe the results in 1-3 min. Not suitable for the crowd Special diseases: Patients with hematopoietic dysfunction, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye.

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