Acid phosphatase staining

Acid phosphatase activity exists in most blood cells. The acid phosphatase in the cytoplasm is hydrolyzed under acidic conditions, and then reacted with lead nitrate or amine sulfate to form a yellow or brown yellow lead sulfate precipitate. Staining reaction of normal blood cells: granulocytes, monocytes, lymphocytes, megakaryocytes, platelets, plasma cells, macrophages are positive. Basic Information Specialist classification: growth and development check classification: microscopy Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Red blood cells and erythroleukemia showed unilateral positive reaction in the nucleus; multiple myeloma cells, acute monocytic leukemia, malignant histiocytosis cells, hairy cell leukemia, T lymphocytic leukemia, etc. were strong Positive reaction. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Normal value Positive granulocytes, T lymphocytes, plasma cells, megakaryocytes, platelets, young red blood cells, and mononuclear-macrophage system cells. Clinical significance Acid phosphatase staining can help identify Gaucher cells (positive reaction) and Niemann-Pick cells (negative reaction); red blood cells and erythroleukemia show unilateral positive reaction to the nucleus; multiple myeloma cells Acute monocytic leukemia, malignant histiocytosis cells, hairy cell leukemia, and T lymphocytic leukemia all showed strong positive reactions. Positive results may be diseases: multiple myeloma, monocytic leukemia, adult T-cell leukemia considerations Acid phosphatase staining, if necessary, add L-tartaric acid inhibition test, that is, acid phosphatase staining is inhibited by tartaric acid; otherwise, it is not inhibited by tartaric acid, resulting in an insoluble red precipitate, which is localized in the cytoplasm. This is important for the differential diagnosis of hairy cell leukemia (L-tartaric acid inhibition test is still positive), while multiple myeloma cells, acute monocytic leukemia cells, malignant histiocytosis cells can be inhibited by L-tartaric acid The positive reaction was significantly weakened. Inspection process Fresh bone marrow tablets or blood samples were fixed in cold methanol-acetone buffer for 30 s, rinsed with distilled water for 5-6 times, and air-dried. The solution was placed at 37 ° C for 45 to 60 minutes and washed with water. The cells were counterstained with hematoxylin or methyl green stain for 1 to 6 min, washed with water, and then examined by microscopy for positive control (ie, acid phosphatase staining): the staining process was the same as above, and 0.1 mol/L acetate buffer was used instead of acetic acid-lithic acid. Buffer. Not suitable for the crowd no. Adverse reactions and risks no.

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