gonococcal culture

Neisseria gonorrhoeae can form round, convex, moist, smooth, translucent or off-white colonies after 24 to 48 hours of incubation on polymyxin B blood agar (a commonly used medium). If the culture is continued, the colony volume is increased, the surface is rough, and the edges are shrunk. If the above situation is manifested, it is initially determined to be positive for gonococcal bacteria, but further determined that an oxidase test or a sugar fermentation test is required. Basic Information Specialist classification: infectious disease inspection and inspection classification: other inspection Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: Normal when negative. Positive: Positive for judging male acute gonococcal urethritis, ie male acute gonorrhea, female gonorrhea and other diseases. Tips: Keep a normal mindset. Normal value Sterile growth, negative. Clinical significance Abnormal results: positive, used to determine male acute gonococcal urethritis, namely male acute gonorrhea, female gonorrhea and other diseases. Need to check the crowd: male, female. Positive results may be diseases: acute gonorrhea, gonorrhea, gonococcal urethritis considerations Inappropriate people: generally no special population. Pre-inspection contraindications: Insulation and moisturization in time for inspection, timely inoculation (within 30 minutes), the medium should be inoculated after incubation. Requirements for inspection: First, the bacteria grows slowly, the nutrient requirements are high, and it needs to grow well under 5% CO2 environment--this is the premise different from other Neisseria. Next is oxidase +, touch enzyme +, other biochemical reactions are carried out by enzymatic method, sugar biochemical tubes for non-fermenting bacteria: glucose, maltose, lactose, sucrose, fructose, others must be used for nitrate and DNA tests. Inspection process Do not rub hard when smearing to prevent cell rupture and deformation. The thickness of the smear should be appropriate. If the smear is too thin, there will be almost no cells in the smear, which will cause false negatives; if the smear is too thick, the discoloration is not easy to clean, which makes the various components difficult to distinguish. Some bacteria in the female reproductive tract, such as Acinetobacter, are very similar to Neisseria gonorrhoeae, often causing false positive results in smears. Direct smear results in pharyngeal and rectal specimens are also often unreliable due to masking of normal flora or various bacteria. Specimens should be inoculated immediately in the culture medium. Commonly used media in foreign countries are Thayer-Martin medium and modified Stuart transport medium. Domestically developed media have also been developed, such as blood agar or chocolate agar supplemented with polymyxin E (25 μg/mL). In the initial separation, a 5% carbon dioxide environment (candle) is used, and the culture temperature is preferably 36 ° C, and the relative humidity is 80% or more. The results were observed in 24-48 hours. Not suitable for the crowd Generally there are no people who are not suitable. Adverse reactions and risks Generally no adverse reactions.

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