Bacteriological examination of pus and wound infection specimens

Pus and wound secretions are the most common in the process of infection. Pus and wound secretion bacterial culture, for the diagnosis of pathogenic bacteria, pus and wound secretion bacteria in the pathogens are exogenous and endogenous. There are many types of bacteria that can be detected from pus and wound secretions. The most preferred pathogens are Staphylococcus aureus and Streptococcus pyogenes, followed by Pseudomonas and Enterobacteriaceae. Specimens were collected and tested by direct smear, common bacterial culture and anaerobic culture. Basic Information Specialist classification: skin examination classification: pathogenic microbiological examination Applicable gender: whether men and women apply fasting: not fasting Tips: Strictly follow the doctor's advice. Normal value In normal human tissues and organs, there is no purulent infection, no bacterial growth in culture, and no bacteria are found in the direct smear. Clinical significance All wounds can be contaminated with bacteria, but infections do not necessarily occur. Therefore, the isolated bacteria should be collected according to the condition of the wound, the amount of bacteria and the number of species, the collection process of the specimen, the immunity of the body and the use of antibiotics. Factors to analyze and judge. Bacteria that may be found in specimens of pus and wound infections. Gram-positive cocci Staphylococcus, streptococcus, Streptococcus pneumoniae, four-cocci. 2. Gram-negative cocci B. catarrhalis, Neisseria gonorrhoeae, Neisseria meningitidis, Dry cocci, Yellowococcus. 3. Gram-positive bacilli Tetanus cloacae, Bacillus anthracis, Clostridium perfringens, Corynebacterium diphtheriae, Corynebacterium diphtheriae, Mycobacterium tuberculosis. 4. Gram-negative bacilli Escherichia coli, Pseudomonas aeruginosa, Proteus, Enterobacter aerogenes, Yersinia pestis, Francisella tularensis, Klebsiella pneumoniae, Anaerobe. 5. Other Actinomycetes, Actinobacter serrata, Candida albicans. Most traumatic traumas have bacterial infections, especially Staphylococcus aureus infections. Chronic suppurative infections are often mixed with Staphylococcus aureus and Streptococcus, and can also be mixed with Enterobacter, Proteus, Pseudomonas, and Bacillus subtilis. Staphylococcus aureus is common in sputum, sputum and subcutaneous abscesses on the skin. Staphylococcus aureus is common in specimens of patients with acute osteomyelitis, followed by hemolytic streptococcus, Streptococcus pneumoniae, and Enterobacter. Bone tuberculosis caused by bacilli is also common. Otitis media, mastitis are caused by Staphylococcus aureus, cellulitis, erysipelas, tonsillitis, posterior pharyngeal abscess, often caused by a mixed infection caused by Streptococcus pyogenes and Staphylococcus. Burning wound pus is most common with Pseudomonas aeruginosa. Bacillus anthracis causes skin anthrax. Tetanus can cause tetanus, and Clostridium perfringens can cause gas gangrene. Cholecystitis and appendicitis are often mixed infections caused by aerobic and anaerobic bacteria. Precautions Before the test: Please follow the doctor's advice. Inspection process Checking steps: 1. First clean the contaminated bacteria on the surface of the lesion with sterile saline. 2. For the ulcerated abscess, the pus and the deep secretion of the lesion are generally taken as a sterile cotton swab, while the fistula is taken as a sterile method and placed in a sterile test tube for examination. 3. For the unruptured abscess, it is best to use 2.5% to 3% iodine and 75% alcohol to disinfect the affected part of the skin, and then use a sterile syringe to extract pus and secretions, or use a sterile cotton swab when cutting the pus. take. 4. Sometimes the innermost dressing with pus can be placed in a sterile dish for inspection. 5. For the specimens of actinomycetes, use sterile cotton swab to squeeze the fistula, select the “sulfur-like particles” in the pus out of the test tube, and put it into the test tube. You can also insert the sterile gauze into the fistula and take it out the next day. Send inspection. Not suitable for the crowd No taboos. Adverse reactions and risks Generally no complications and harm.

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