Epidemic hemorrhagic fever virus antibody

Shortly after the infection of the epidemic hemorrhagic fever virus, EHF-IgM was detected in the serum and peaked within 7 to 10 days. EHF-IgG appears only 2 weeks after onset and can last for several years. The determination of EHF-IgM is of great value in the diagnosis of early epidemic hemorrhagic fever infection. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: EHF-IgM was negative and EHF-IgG was negative and normal. Positive: EHF-IgM is positive in epidemic hemorrhagic fever. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. The alcohol content in the blood directly affects the test results. Normal value Negative: EHF-IgM negative, EHF-IgG negative. Clinical significance EHF-IgM is positive in epidemic hemorrhagic fever. Positive results may be diseases: epidemic hemorrhagic fever, dengue hemorrhagic fever considerations Preparation before inspection: 1. Please inform the doctor about the recent medication and special physiological changes before the test. 2, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 3. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Requirements for inspection: When you draw blood, you should relax your mind, avoid the contraction of blood vessels caused by fear, and increase the difficulty of blood collection. Inappropriate people: generally no special population. Inspection process 1, laboratory materials blood. 2, the principle of determination of epidemic hemorrhagic fever virus antibody Indirect immunofluorescence: a cell smear infected with EHF virus is used as a target antigen tablet, and serum to be tested is added. After the reaction, the cells are stained with fluorescein (FITC)-labeled anti-human IgM, and cells are colored according to the presence or absence of specific fluorescence. Interpret the results. 3, reagents Antigen tablets and FITC-labeled anti-human IgM are commercially available as ready-to-use reagents. The principle of preparation of antigenic tablets is to take a culture of VeroE-6 cells infected with EHF virus to prepare a dispersed cell suspension, which is added dropwise to a sterilized 10-well coated glass slide, and a humidified box of 5% carbon dioxide is placed. Under the environment, continue to culture for 16 ~ 18h, the cells are attached to the slide, followed by washing, acetone fixed, dry sealed and stored at -20 ° C. A smear of uninfected VetoE-6 cells was prepared in the same manner for comparison. 4, the operation method (1) The test serum was diluted with 5 mmol/L PBS (pH 7.2), and then added dropwise to the antigen sheet, and then placed in a humidified box at 37 ° C for 45 minutes. (2) Washing 3 times with PBS, immersing for 3 min each time, shaking for 2 min, drying, adding 0.1 g/L of Evans blue-PBS formulated FITC-labeled anti-human IgM, incubating with the same method, washing and drying. (3) Using a non-fluorescent polyvinyl alcohol-glycerol gel-sealed piece, observed under a fluorescence microscope, where the point or sheet-like specific fluorescence in the cytoplasm is positive, according to the number of positive cells and the fluorescence intensity (+) ~ ( ++++), and the reciprocal of the highest dilution of the positive serum is the antibody titer in the test specimen. Not suitable for the crowd Those who do not have an indication for examination should not do this check. Adverse reactions and risks Generally no complications and harm.

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