Sodium hippurate hydrolysis test

The sodium urate hydrolysis test is based on the principle that certain bacteria may have a horse uric acid hydrolase, which can hydrolyze horse uric acid to benzoic acid and glycine, and benzoic acid is combined with ferric chloride reagent to form iron benzoate precipitate. Basic Information Specialist classification: Infectious disease inspection and classification: pathogenic microorganism inspection Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Positive: Abnormal results suggest suppurative infections: tonsillitis, erysipelas, cellulitis, lymphangitis, sepsis, etc.; scarlet fever, rheumatic fever, glomerulonephritis after streptococcal infection, neonatal sepsis and meningitis. Tips: Pay attention to normal eating habits and pay attention to personal hygiene. Normal value The type and proportion of the flora in the body are normal, and the human body is in a dynamic balance. Clinical significance The use is mainly used for the identification of group B streptococcus. Group A Streptococcus peptide sensitivity test (+). Group B Streptococcus CAMP test (+), sodium urate hydrolysis test (+), group D Streptococcus bile esculine test (+). Abnormal results: purulent infection tonsillitis, erysipelas, cellulitis, lymphangitis, sepsis, etc.; scarlet fever, rheumatic fever, glomerulonephritis after streptococcal infection; neonatal sepsis and meningitis. People who need to be examined: those with the above symptoms. Positive results may be diseases: cellulitis, erysipelas, rheumatic fever, scarlet fever, tonsillitis, acute lymphangitis, sepsis, meningitis considerations Inappropriate crowd: No. Forbidden before examination: Pay attention to normal eating habits and pay attention to personal hygiene. Requirements for inspection: Actively cooperate with the doctor. Inspection process Medium: sodium urate medium. Reagent: 12 g of ferric chloride was dissolved in 100 ml of 2% hydrochloric acid. METHODS: The pure culture was inoculated into sodium hyaluronic acid medium, and cultured in a 37-degree incubator for 2-4 days. The water lost in the culture solution was made up with distilled water to the original amount. The supernatant was centrifuged to absorb 0.8 ml of the supernatant, and the reagent was added. 0.2ml, mix well immediately, after 10-15min, observe the results. If there is a precipitate that does not disappear for a long time, it is positive for the sodium urate hydrolysis test. Note: Do not use too much reagent, otherwise iron benzoate may be redissolved. Not suitable for the crowd Nothing is suitable for the crowd. Adverse reactions and risks Generally no complications and harm.

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