salivary aldosterone

Aldosterone is the most important mineralocorticoid secreted by the adrenal cortical globular band. Normal people have very little secretion and the body degrades rapidly. Aldosterone is an important hormone that maintains the body's water and electrolyte balance. Its physiological role is to promote the reabsorption of sodium by the renal distal convoluted tubule epithelial cells and promote potassium excretion. The secretion of aldosterone has a diurnal secretion rhythm similar to cortisol, which is high in the morning and low in the night, and is also regulated by the renin-angiotensin system and blood electrolytes. The secretion of aldosterone is increased in the standing position than in the supine position, and its plasma concentration also changes accordingly. Therefore, blood samples are generally taken at around 8 am in the clinic for examination. The salivary aldosterone concentration is similar to the plasma concentration. Basic Information Specialist classification: oral examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Reduced in Edison's illness, stillbirth, miscarriage, malignant mole, 18-hydroxysteroid dehydrogenase deficiency, 18-hydroxylase deficiency, etc. Normal value: Salivary aldosterone: 15.0-39.8pg/ml Above normal: Increased in primary aldosteronism, congestive heart failure, cirrhosis, 17-alpha hydroxylase deficiency, polycystic kidney disease, adrenocortical carcinoma, renin secretory tumor, multiple births, pregnancy-induced hypertension syndrome. negative: Positive: Tips: Limiting the diet of sodium and potassium can make the measurement results higher. Normal value 15.0 to 39.8 pg/ml. Clinical significance Reduction: seen in Edison's illness, stillbirth, miscarriage, malignant mole, 18-hydroxysteroid dehydrogenase deficiency, 18-hydroxylase deficiency, etc. Elevation: seen in primary aldosteronism, congestive heart failure, cirrhosis, 17-alpha hydroxylase deficiency, polycystic kidney disease, adrenocortical carcinoma, renin secretory tumor, multiple births, pregnancy-induced hypertension syndrome. High results may be diseases: primary aldosteronism, congestive heart failure, polycystic kidney disease, pregnancy-induced hypertension syndrome, cirrhosis, malignant moles 1. Specimens are kept with serum cortisol. However, the diet should control sodium and potassium. The standard diet is: sodium 100mmol/d and potassium 60-100mmol/d. 2, taking certain drugs can affect the results of aldosterone determination, such as oral contraceptives, estrogens, furosemide, angiotensin and other drugs can make the test results higher; taking risepone, dehydrocorticosterone, propranolol , methyldopa and the use of heparin, etc., can reduce the results. 3. Limiting the diet of sodium and potassium can make the measurement result higher. Inspection process The method is divided into three steps, namely antigen-antibody reaction, B and F separation, and radioactivity determination. 1. Antigen and antibody reaction: The specimen (non-labeled antigen), labeled antigen and antiserum are sequentially dosed into a small test tube, and allowed to stand at room temperature (15-30 ° C) for 24 hours to fully compete for binding. 2, B, F separation: a variety of separation techniques, commonly used precipitation method. 1 second antibody precipitation method: also known as diabody method, after the test antigen specifically reacts with the first antibody, the corresponding second antibody is added, so that the formed antigen-first antibody-second antibody complex is co-precipitated. The labeled antigen B is separated from the free antigen F by centrifugation. This method is a specific precipitation, complete separation, low non-specific binding. However, the amount of the second antibody is large and the cost is high. In addition, the concentration of the specimen and the presence or absence of the anticoagulant can affect the results to some extent. 2 Polyethylene glycol (PEG) precipitation method: the protein is in an isoelectric point state, and the hydration layer is destroyed to cause protein precipitation. The advantage of this method is that PEG is convenient to prepare, inexpensive, and rapid to separate. The disadvantage is that there are many non-specific precipitates and the separation is incomplete. 3Second antibody-polyethylene glycol precipitation method: This method not only has the advantage of rapid precipitation of PEG method, but also maintains the effect of specific precipitation of second antibody, reduces the amount of second antibody, and reduces the concentration of PEG, so that non-specific precipitation Reduced material. 4 Activated carbon adsorption method: the free part of small molecules is adsorbed by the surface activity of activated carbon. For example, a layer of dextran is coated on the surface of the activated carbon to make a mesh having a certain pore diameter on the surface, thereby allowing small molecules of free antigen or hapten to escape and being adsorbed, while the macromolecular complex is excluded. After the antigen and the antibody are reacted, the dextran-activated carbon is added and allowed to stand for 5 to 10 minutes, so that the free antigen is adsorbed on the activated carbon particles, and the particles are precipitated by centrifugation, and the supernatant contains the labeled antigen. 3. Determination of radioactivity: After separation of B and F, the radioactivity can be determined. There are two types of measuring instruments: a liquid scintillation counter (measuring beta rays) and a crystal scintillation counter (measuring gamma rays). The unit of counting is the number of electrical pulses output by the detector in units of cpm (number of pulses/min). A standard curve is required for each measurement, and the different concentrations of the standard antigen are plotted on the abscissa, and the corresponding radioactivity measured is plotted on the ordinate. The radioactivity may be optionally B or F, and the calculated values ​​B/B+F, B/F or B/Bo may also be used. Specimens should be determined in duplicate, the average value is taken, and the corresponding antigen concentration is detected on the standard curve. Not suitable for the crowd no. Adverse reactions and risks no.

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