Glycated serum protein

The glycated serum protein is a non-enzymatic glycation reaction between serum glucose and the N-terminal amino group of albumin and other serum protein molecules to form a polymer ketone amine structure. Because plasma proteins, especially albumin, have a short half-life (19 days), they can reflect the more recent effects of diabetes treatment, and understand the blood sugar levels of diabetes control for 1 to 2 weeks. Glycosylated serum proteins have been reported to be well correlated with GHb. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Warm reminder: pH value, reaction temperature and reaction time have great influence on this test and must be strictly controlled. Normal value 1.9 ± 0.25 mmol / L. Clinical significance 1, due to the short half-life of serum protein, this test can effectively reflect the patient's blood glucose levels in the past 1-2 weeks. 2. This test is not affected by the fluctuation of temporary blood glucose concentration, so it provides a good indicator for the diagnosis of clinical diabetics and the study of long-term blood glucose control. Comparison of consecutive test results before and after the same patient is more valuable. Precautions 1. DMF can be synthesized by itself. The method is to weigh 90g of anhydrous D-glucose, 58g of morpholine, and add 1L of distilled water. After dissolving, it is stirred on a water bath of 60-70 °C to start a yellow paste, and the color gradually deepens. After 20 min, the water bath was removed and 18 g of malonic acid was slowly added. The entire addition process took more than 10 min. The bath was re-watered and the temperature was raised to 80 ° C, stirring was continued, and the color changed from yellow-green to amber. After 10 min, 70 ml of absolute ethanol was added, maintained at 75 ° C for 30 min, and then 70 ml of acetone was added. At this point, crystallization is observed, which is DMF. Put in a refrigerator at 4 ° C overnight. The crystals were collected and recrystallized three times with absolute ethanol to purify the product and dried. The melting point of 146 ~ 147 ° C, the molecular formula C10H19O6N, molecular weight 249D. 2. The pH value, reaction temperature and reaction time have great influence on the test and must be strictly controlled. 3. The non-enzymatic glycation reaction of serum proteins can form a ketone amine structure. DMF can also form a 1-deoxy-1-amino-2-keto ketoamine structure from an oxygen ring structure through structural rearrangement under appropriate pH and temperature conditions. It is therefore reasonable to use it as a standard reference. 4. The freeze-thawed serum protein is used as a standard, and the determination result is more stable. Because the results measured with different standards are not exactly the same, it is best to establish a laboratory reference value. Inspection process The measuring tube was added with 0.1 ml of serum (plasma) to be tested, and 0.1 ml of distilled water was added to the blank tube, and 4 ml of NBT reagent pre-warmed at 37 ° C was added, mixed, placed in an accurate water bath at 37 ° C for 15 min, and cooled by running water (less than 25 ° C). After 15 minutes of cooling, the wavelength of the spectrophotometer was 550 nm, and the optical path of the 10 mm optical path was zeroed by blank, and the absorbance of the measuring tube was read. The results were found from the standard curve. Reported with fructosamine mmol/L. Note: 1. DMF can be synthesized by itself. The method is to weigh 90g of anhydrous D-glucose, 58g of morpholine, and add 1L of distilled water. After dissolving, it is stirred on a water bath of 60-70 °C to start a yellow paste, and the color gradually deepens. After 20 min, the water bath was removed and 18 g of malonic acid was slowly added. The entire addition process took more than 10 min. The bath was re-watered and the temperature was raised to 80 ° C, stirring was continued, and the color changed from yellow-green to amber. After 10 min, 70 ml of absolute ethanol was added, maintained at 75 ° C for 30 min, and then 70 ml of acetone was added. At this point, crystallization is observed, which is DMF. Put in a refrigerator at 4 ° C overnight. The crystals were collected and recrystallized three times with absolute ethanol to purify the product and dried. The melting point of 146 ~ 147 ° C, the molecular formula C10H19O6N, molecular weight 249D. 2. The pH value, reaction temperature and reaction time have great influence on the test and must be strictly controlled. 3. The non-enzymatic glycation reaction of serum proteins can form a ketone amine structure. DMF can also form a 1-deoxy-1-amino-2-keto ketoamine structure from an oxygen ring structure through structural rearrangement under appropriate pH and temperature conditions. It is therefore reasonable to use it as a standard reference. 4. The freeze-thawed serum protein is used as a standard, and the determination result is more stable. Because the results measured with different standards are not exactly the same, it is best to establish a laboratory reference value. Not suitable for the crowd no. Adverse reactions and risks no.

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