proline peptidase

Proline peptidase (PLD) or purine peptidase, an iminopeptidase widely distributed in various tissues and cells of human body, catalyzes the hydrolysis of C-terminal proline or hydroxyproline 2 peptide. Recycling of proline during collagen synthesis and cell growth plays an important role. Its blood level changes are closely related to the degree of liver damage and chronic liver disease. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: No clinical significance. Normal value: Proline peptidase (modified colorimetric method): 912.9-1301.9U/L Above normal: Acute hepatitis, chronic liver disease, alcoholic liver disease, severe hepatitis. negative: Positive: Tips: You can't eat breakfast in the morning when you take a blood test. It is best not to smoke, drink, stay up late before taking blood. Pay attention to rest. Normal value The plasma color PLD activity of 100 healthy people was 1107.4±194.5 U/L by modified colorimetry. Males (1115.3±208.1 U/L) were slightly higher than females (1089.9±182.4 U/L), but there was no significant difference (P>0.05). Clinical significance Abnormal results: 1. Acute hepatitis Serum PLD activity was significantly elevated in patients with acute hepatitis and was positively correlated with PLD. Single-dose injection of CCL4-induced acute liver injury in rats showed that the serum value of PLD was significantly higher (3000±190U/L) at 3h after injection than that of the normal control group (2450±40U/L), and peaked at 24h (3780±230U/L). 72h recovery is normal, at this time ALT, AST is still higher than normal. Hu Darong et al reported that plasma PLD activity (2746.1±985.1 U/L) was significantly higher in patients with acute viral hepatitis than in the normal control group (1107.4±194.5 U/L), which was the same as the ALT abnormal rate in the same group, all of which were 93.8%. It indicates that PLD is a sensitive index reflecting acute liver injury and recovery. The increase of serum PLD in acute hepatitis may be related to the necrosis of hepatocytes and the release of PLD into the blood. 2, chronic liver disease Foreign data show that serum PLD is elevated in patients with chronic hepatitis and cirrhosis, while ALT is more normal in the same group. Experimental study on chronic toxic liver injury induced by CCL4 in rats showed that PLD fluctuated within the normal range in the third week, but there was an upward trend, and histology could see the development of rat liver fibrosis; at 6 weeks, PLD reached At the peak, liver tissue showed extensive recurrent fibrosis; at 12 weeks, PLD decreased and approached normal, while histology showed cirrhosis. Hu Darong et al reported that plasma PLD (1944.4±213.0 U/L) in patients with chronic liver disease was significantly higher than that in the normal control group, and the change was not significantly correlated with ALT (r=0.1630, P>0.05), and the positive rate (90.1%) was significantly higher. At ALT (P>0.05), the highest PLD was observed in patients with cirrhosis (2497.4±259.4 U/L), the positive rate was 90.5%, and the ALT was only 4.8% (P>0.01). The results show that PLD is more practical in reflecting chronic liver disease and progressive liver fibrosis, especially ALT and normal. Chronic liver disease such as ALT is normal, and PLD is significantly increased, the possibility of cirrhosis should be considered. Therefore, PLD detection can be used as an indicator for diagnosing or preventing cirrhosis. The increase of PLD in patients with chronic liver disease may be related to the enhancement of PLD activity in liver cells, accelerated degradation of intracellular collagen, and increased proline content, which increases the synthesis of collagen between cells. 3. Alcoholic liver disease Brosset et al. Correlation between plasma PLD levels and liver biopsy in 53 patients with alcoholic liver disease found that patients with alcoholic liver disease had elevated blood PLD, and pathology showed reversible liver fibrosis and irreversible liver fibrosis, but no Acute alcoholic liver injury manifestations; cirrhosis with acute alcoholic liver injury, PLD levels were higher than those with reversible and irreversible liver fibrosis. Blood PLD levels were not associated with ALT in patients with alcoholic liver disease, but were associated with AST (r=0.505, P>0.001). This is related to the hepatic pathological damage of patients with alcoholic liver disease mainly in the mitochondria, while in liver cells 80% AST. The conclusion of the distribution of mitochondria is consistent. 4, severe hepatitis Myara et al reported that 20 patients with severe hepatitis had a mild increase in blood PLD, with significantly higher elevations. Hu Darong and other people think that in patients with severe hepatitis, the PLD and ALT levels are higher than those in healthy people, and the changes are positively correlated (r=0.965, P>0.01), but the significantly elevated patients are only seen in a few patients, and the abnormal rate is also No significant difference. Like ALT in the blood of patients with severe hepatitis, PLD also has the phenomenon of "enzyme separation." High results may be diseases: liver disease precautions Note before inspection: 1, can not eat breakfast in the morning of blood tests. 2, it is best not to smoke, drink, stay up late before taking blood. Pay attention to rest. Requirements for inspection: 1. The doctor should be informed of the drug history. 2. If you have any discomfort during the examination (such as dizziness, etc.), you should inform the doctor. Not suitable for people: Nothing is suitable for the crowd. Inspection process 1. Drain 3 ml of blood, place it in an anticoagulant tube, place it in a 4°C micro-top centrifuge, carefully remove the upper yellow liquid into a new 1.5 ml centrifuge tube, and transfer an appropriate amount of microliter for protein quantitative detection. Store in the ice trough or put it in the -70 °C refrigerator; take 3 ml of blood, place it in the storage tube, let it stand at room temperature, put it into a 4 °C micro table centrifuge, and remove the upper yellow liquid to the new 1.5 The milliliter centrifuge tube is used for quantitative protein detection; 1 ml of blood is taken, placed in an anticoagulant tube, placed in a 4 ° C micro-top centrifuge, centrifuged carefully, the upper yellow liquid is carefully removed, pre-cooled non-ionized water is added, and the vortex is vortexed. Immediately placed in an ice bath or placed in a -70 ° C freezer for storage. 2. Prepare the above sample to be tested and place it in the ice trough. Set the spectrophotometer at a wavelength of 515 nm and set it to zero. Put into the spectrophotometer to detect, this is the background air control. Not suitable for the crowd no. Adverse reactions and risks no.

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