monoamine oxidase

Monoamine oxidase (MAO) is an enzyme that reflects liver fibrosis. It can be divided into two types, mitochondria, which are found in tissues such as liver and kidney. FAD is a coenzyme and participates in the catabolism of catecholamines. The other type, which is found in connective tissue, is an extracellular enzyme that contains pyridoxal phosphate without FAD and acts only on primary amines. The MAO in serum and MAO in connective tissue are similar in nature, which can promote the maturation of connective tissue. In the process of collagen formation, it participates in the formation of bridging in the final stage of collagen maturation, and combines collagen and elastin. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: No clinical significance. Normal value: Monoamine oxidase: 12-40U/ml Above normal: The liver is hard. negative: Positive: Tips: When liver cirrhosis, serum MAO activity is often significantly increased, the positive rate can be as high as 80% or more. Normal value 12 to 40 U/ml (12,000 to 40000 U/L). Clinical significance The MAO in serum and MAO in connective tissue are similar in nature, which can promote the maturation of connective tissue. In the process of collagen formation, it participates in the formation of bridging in the final stage of collagen maturation, and combines collagen and elastin. Clinically, serum MAO is mainly used to diagnose cirrhosis. When cirrhosis occurs, serum MAO activity is often significantly increased, and the positive rate can be as high as 80% or more. High results may be diseases: liver cirrhosis, cirrhosis of the elderly, liver fibrosis 1. It is not easy to dissolve benzylamine azo-β-naphthol, and it is necessary to gradually wash the pigment in a hot water bath until only black insoluble matter remains. 2. To fully extract the benzaldehyde compound into cyclohexane, it must be placed in a 37 ° C water bath for 30 min and stirred 2 times. 3. The absorption peak of the benzaldehyde compound cyclohexane extract is 480 nm. To reduce the interference of bilirubin in serum, the wavelength of 500 nm is used. 4. Cyclohexane and water are not miscible. Before colorimetry, the cuvette must be washed with absolute ethanol and ether to make it dry and then colorimetrically. Inspection process The monoamine oxidizing enzyme acts mainly on the -CH-NH2 group and, under the participation of oxygen, catalyzes the oxidation of a monoamine to form the corresponding aldehyde, ammonia and hydrogen peroxide. Using benzaldehyde azo-β-naphthol as a substrate, MAO catalyzes the formation of benzaldehyde azo-β-naphthol (ammonia and hydrogen peroxide) with the participation of O2 and H2O, which is directly extracted with cyclohexane. Colorimetric determination. Not suitable for the crowd no. Adverse reactions and risks no.

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