amylase isoenzyme

The agarose electrophoresis method can divide the amylase isoenzyme into two types, namely, S type (saliva type) and P type (pancreatic type). Amylase isoenzyme assays are helpful in identifying pancreatic diseases. Basic Information Specialist classification: Digestive examination classification: biochemical examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: No clinical significance. Normal value: AMY-P (pancreatic): 8-45U/L AMY-S (saliva type): 8-70U/L Above normal: 1, AMY-P elevated acute pancreatitis. 2. AMY-S raises adenine, kidney failure, and cancer. negative: Positive: Tips: Keep a normal mindset. Normal value Enzyme inhibition method (37 ° C). AMY-P (pancreatic) 8 ~ 45U / L (mainly in the urine). AMY-S (saliva type) 8 ~ 70U / L (mainly in blood). Clinical significance 1, AMY-P elevated acute pancreatitis. 2. AMY-S raises adenine, kidney failure, and cancer. High results may be diseases: precautions for acute pancreatitis 1. Sulfur-starch colorimetric method: (1) Serum and heparin anticoagulated plasma results were consistent, while EDTA-Na2, oxalate, citrate, and sodium fluoride inhibited AMS activity, resulting in low results. (2) The enzyme activity is significantly increased, that is, when the absorbance of the measuring tube is less than half of the absorbance of the blank tube, the substrate should be increased by the dilution factor, or the sample addition amount can be reduced due to the relative shortage of the substrate, and the measurement result is multiplied by the dilution factor. (3) AMS activity in saliva is high and should be prevented from entering. (4) If the starch base liquid appears turbid or floc, suggesting deterioration or contamination, it should be reconstituted. (5) The concentration of iodine solution can affect the measurement results. Generally, the iodine application solution should be reconstituted for more than one month. 2, p-nitrobenzene malt heptasaccharide method: (1) When ΔA/min>0.15, the specimen should be diluted 10 times with physiological saline and the measurement result multiplied by the dilution factor. (2) The kits, components and methods are not the same and should be subject to the instructions. Inspection process Determined by enzyme inhibition method. Not suitable for the crowd no. Adverse reactions and risks no.

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