Denatured globulin (Heinz) body generation assay

The redox dye tar tar blue is added to the blood. After incubation at 37 ° C, HbH precipitates due to oxidative degeneration, is granular, is dyed dark blue, and is uniformly and rapidly dispersed in red blood cells. Buffer: 0.066 mol/L KH2PO 413 parts was added to 0.066 mol/L Na2HPO4B 7 parts to prepare a pH 7.6 solution, and 200 mg of glucose was added to 100 ml of the above solution. This solution is stored at 4 ° C. If the solution is cloud-like, it should be discarded. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Seen in the broad bean disease caused by G-6-PD deficiency, hemolytic anemia caused by primary amino acid quinoline drugs, unstable hemoglobin (Hb) disease and the like. Tips: Choose a digestible high protein diet. Normal value Red blood cells containing more than 5 denatured globin bodies, the normal person accounted for 0% to 28% of the average of 11.9%. The average G-6-PD deficiency was up to 67.8%. Some authors suggested that 32.5% were normal and abnormal boundaries. Clinical significance Glucose-6-phosphate glucose dehydrogenase (G-6-PD) deficiency can reduce the content of reduced glutathione in erythrocytes, followed by the increase of methemoglobin, and finally the formation of denatured globin bodies, which are attached to the cell membrane. A denatured hemoglobin granule, also known as a hemoglobin inclusion body, can be dyed by some basic dyes into stains or blue-black dots. Increased in the broad bean disease caused by G-6-PD deficiency, hemolytic anemia caused by primary amino acid quinoline drugs, unstable hemoglobin (Hb) disease, etc., an average of 67.8% (45% to 92%). Positive results may be diseases: prevalence of faba bean disease 1. The concentration of buffer and acetophenone, the amount of blood added and the holding time have a certain influence on the results, so the standard stress at the time of inspection is consistent. 2. Absorbing with a dropper is to make red blood cells fully contact with oxygen, which is an important step for the formation of denatured globin bodies. 3. The red blood cell ratio of the subject should be adjusted to a level close to normal. Inspection process 1. Anti-fibrin or heparin, EDTA, oxalate anticoagulation, take blood and normal control blood. 2. Take 2 tubes of test tube (7.5cm×1.2cm), add 2ml of acetaminophen buffer to one tube, add 0.1ml of anticoagulant to be tested, add the same amount of acetophenone buffer to the other tube, and then add normal blood. 0.1 ml as a control. 3. The above two tube mixture was sucked several times with a dropper, incubated at 37 ° C for 2 h, then sucked several times with a pipette, and then incubated at 37 ° C for 2 h. 4. Dyeing: Take 1 drop of the above mixture on a glass slide, add 2 to 3 drops of a solution of methyl violet solution, and mix. 5. Cover the coverslip and observe the denatured globin bodies under the microscope after 5-10 min. Not suitable for the crowd no. Adverse reactions and risks no.

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