Denatured globulin (Heinz) body staining assay

Glucose-6-phosphate glucose dehydrogenase (G-6-PD) deficiency can reduce the content of reduced glutathione in erythrocytes, followed by the increase of methemoglobin, and finally the formation of denatured globin bodies, which are attached to the cell membrane. A denatured hemoglobin granule, also known as hemoglobin inclusion body, can complement some basic dyes to dye purple or blue-black dots. Basic Information Specialist classification: growth and development check classification: biochemical examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Seen in the broad bean disease caused by G-6-PD deficiency, hemolytic anemia caused by primary amino acid quinoline drugs, unstable hemoglobin (Hb) disease and the like. Tips: Try to eat less and eat as much as possible, and arrange your diet reasonably. Normal value negative. Clinical significance Glucose-6-phosphate glucose dehydrogenase (G-6-PD) deficiency can reduce the content of reduced glutathione in erythrocytes, followed by the increase of methemoglobin, and finally the formation of denatured globin bodies, which are attached to the cell membrane. A denatured hemoglobin granule, also known as a hemoglobin inclusion body, can be dyed by some basic dyes into stains or blue-black dots. Increased in the broad bean disease caused by G-6-PD deficiency, hemolytic anemia caused by primary amino acid quinoline drugs, unstable hemoglobin (Hb) disease, etc., an average of 67.8% (45% to 92%). Positive results may be diseases: prevalence of faba bean disease 1. In patients with faba bean disease lacking 6-phosphate glucose dehydrogenase, denaturing globin bodies can be observed within 48 hours after onset, and will gradually decrease in the future. 2. The denatured globin bodies are small particles when they are initially formed, and gradually become coarse particles in the future. 3, the methyl violet staining specimen can be fixed and counterstained, and the denatured globin body directly microscopically examined is purple. Inspection process 1. Take 2 drops of ear blood, drop on the concave glass slide, and add 2 drops of the same amount of 5g/L methyl violet solution. 2. Gently mix the blood with the aqueous solution of the blue purple salt at one end of the other slide, and dye it for 20-30 minutes. 3. The blood film is smoked in formaldehyde steam in a 37 ° C water bath for 20 to 30 minutes. 4. Rinse thoroughly with distilled water. 5, 1g / L sand yellow counterstained for 10min. 6. Under the microscope, observe 500 red blood cells with oil mirror, count the red blood cells containing denatured globin bodies, and finally obtain the percentage of red blood cells containing denatured globin bodies. The denatured globin bodies are refractive and round. Small bodies of different sizes, dyed purple (less than 3 μm in diameter). Such small bodies have a tendency to be distributed around the cells, and the number is mostly from 1 to 6. Not suitable for the crowd no. Adverse reactions and risks no.

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