Drug allergy testing

No bacteria were found in normal people's blood, urine, cerebrospinal fluid, pleural fluid, pericardial fluid, and peritoneal fluid. There are three ways in which bacteria are sensitive to antibiotics. 1. Diffusion method agar plus various nutrients required by bacteria, melt the culture medium, pour it into a sterile Petri dish, cool, and condense into a flat surface or plate (plate). At this time, the bacterial solution containing a small number of bacteria is coated on the plate, and the bacteria will be split on the plate after culture. If antibiotic paper is added to the culture medium of the plate in advance, the drug spreads in the medium, which inhibits (Bactericidal) bactericidal effect, forming a suppression circle of not long colonies. The size of the inhibition circle reflects the degree to which an antibiotic inhibits the bacteria. 2. Dilution method commonly used drug sensitivity dilution method can be divided into test tube dilution method and agar dilution method: (1) test tube dilution method first test antibiotics are made a series of multiple dilution, and then each test tube is added to the appropriate dilution of the test bacteria solution, shake After observation, the tube with the minimum antibacterial concentration (MiC) of the antibacterial drug is the sensitivity of the test strain. (2) Agar dilution method: Add different doses of antibacterial drugs to quantitative agar medium that has been melted and cooled to 45 ° C, mix well, and pour into sterile plates, that is, the medium containing decreasing drug concentration. Bacteria are grown on this medium. After culture, the growth of the tested bacteria is observed. The lowest drug inhibits the growth of bacteria, that is, the minimum inhibitory concentration (Mic). 3． Automatic analysis in France using AUTOBAC instrument for bacterial drug sensitivity determination. The instrument uses the ring drug low tablets, culture shaker and photometer host to test the data. According to drug resistance, medium resistance, sensitivity, and the minimum inhibitory concentration are all print it out. This instrument is only suitable for the determination of aerobic bacteria and facultative anaerobic bacteria, not for the testing of obligate anaerobic bacteria and mycobacterium tuberculosis. The test results reflect the degree of bacteriostasis of an antibiotic to this bacterium, which is of great significance to guide physicians in the correct use of drugs. Patients with severe inflammatory reactions and ineffective medication experience can perform this test.

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