Hemoglobin solubility

HbF is more alkaline and acid resistant than normal hemoglobin (HbA). Therefore, after blood tablets are eluted with acid, HbF-containing red blood cells are not discolored due to acid resistance and can be stained red by eosin. Red blood cells containing HbA or only a small amount of HbF are eluted by the acid, leaving only a shadow of the red blood cell membrane. Severe & beta; -thalassemia blood sheets are similar to neonatal; mild & beta; -thalassemia has only a few eosinophil-positive red blood cells, and the staining varies.

The material in this site is intended to be of general informational use and is not intended to constitute medical advice, probable diagnosis, or recommended treatments.