Sputum secretory IgE

SIgE is produced similarly to SIgA. It is produced by plasma cells in the lamina propria of the respiratory tract and is distributed in mucosal tissues and exocrine fluids. These sites are the entry sites of allergens and sites of allergic reactions. The principle of ELISA is based on the solid phase of the antigen or antibody and the enzyme labeling of the antigen or antibody. The antigen or antibody bound to the surface of the solid phase carrier maintains its immunological activity, and the enzyme or conjugate of the antigen or antibody retains both its immunological activity and enzyme activity. During the measurement, the test specimen (the antibody or antigen in the assay) and the enzyme-labeled antigen or antibody are reacted with the antigen or antibody on the surface of the solid phase carrier in different steps. The washing method is used to separate the antigen-antibody complex formed on the solid phase carrier from other substances, and finally the amount of the enzyme bound to the solid phase carrier is proportional to the amount of the test substance in the specimen. After the substrate of the enzyme reaction is added, the substrate is catalyzed by the enzyme to become a colored product. The amount of the product is directly related to the amount of the test substance in the specimen, so qualitative or quantitative analysis can be performed according to the depth of coloration. Due to the high catalytic efficiency of the enzyme, the results of the reaction can be greatly amplified, thereby enabling the method to achieve high sensitivity.

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