erythrocyte pyruvate kinase activity

Pyruvate kinase is a tetramer and is an important enzyme that provides red blood cell energy. In the absence of this enzyme, red blood cells are destroyed by energy consumption. Colorimetry is used. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Erythrocyte pyruvate kinase activity is reduced, found in hereditary pyruvate kinase deficiency, certain acquired pyruvate kinase defects (such as granulocyte leukemia, myelodysplastic syndrome). Normal value: Red blood cell PK: 10.1-20U/gHb Above normal: No relevant information. negative: Positive: Tips: After 8 pm on the day before the medical examination, you should start fasting for 12 hours, so as not to affect the test results. Normal value a) colorimetric method 10.1 ~ 20U / gHb; b) Fluorescent spot method is negative. Clinical significance This test can be used as a diagnostic test for pyruvate kinase deficiency. Erythrocyte pyruvate kinase activity is reduced, found in hereditary pyruvate kinase deficiency, certain acquired pyruvate kinase defects (such as granulocyte leukemia, myelodysplastic syndrome). Low results may be diseases: myelodysplastic syndrome considerations Before inspection: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. After inspection: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The examination process: blood is drawn, blood is taken for examination, and venous blood is generally taken, and blood is drawn by a doctor or a nurse. The amount of blood drawn is determined according to the difference of the test content and the number of items. The blood volume is usually 2-20 ml, and the maximum is not more than 50 ml. Then the doctor performs protein color test. Not suitable for the crowd People with reduced hematopoietic function such as leukemia, various anemia, myelodysplastic syndrome, or people with thrombocytopenia should pay attention to blood draw, and should not take more or more blood. Adverse reactions and risks 1. After the blood is drawn, do not press the needle hole to avoid subcutaneous hematoma. If there is a small piece of bruise in the blood, it is slightly tender, please don't panic, you can do hot compress after 24 hours to promote the absorption of blood. The general small amount of congestion will gradually absorb in 3 to 5 days and the color will become lighter and return to normal. 2. After the blood draw, symptoms such as dizziness, vertigo, fatigue, etc. should be immediately supine, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved.

The material in this site is intended to be of general informational use and is not intended to constitute medical advice, probable diagnosis, or recommended treatments.