Insulin antibodies

There are two cases of insulin antibody, one in patients treated with exogenous insulin, mainly related to the purity of insulin preparations, one in patients who have never received insulin treatment, called insulin autoantibodies. Basic Information Specialist classification: growth and development check classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: diabetes. Tips: Try to eat less and eat as much as possible, and arrange your diet reasonably. Normal value negative. Clinical significance Insulin antibodies are very important for the diagnosis, differential diagnosis and treatment of diabetes and hypoglycemia. (1) Early detection of type 1 diabetes In normal people, if insulin antibodies are found in the blood, they are prone to type 1 diabetes. Insulin autoantibodies may be produced by the destruction of β-cells, so the detection of insulin autoantibodies can be used as a marker of autoimmune β-cell damage and can be used for early detection and prevention of type 1 diabetes. (2) Diagnosing insulin resistance and guiding the treatment of diabetes. The presence of insulin antibody in the blood is an important cause of insulin resistance. In the process of insulin therapy, diabetic patients may develop insulin resistance due to the production of insulin antibodies, which is manifested by an increase in insulin dosage. Blood sugar control is not ideal. Insulin antibodies should be tested at this time. If there is a positive or increased titer, it can be used as an objective basis for insulin resistance. Switching to one-component insulin, high-purity insulin, and discontinuation of insulin, oral hypoglycemic agents, or the use of glucocorticoids all help reduce insulin antibody concentrations and improve insulin resistance. (3) Insulin autoimmune syndrome Since Hirata was first reported in 1970, domestic and international reports have increased year by year. Insulin autoimmunesyndrome (IAS) is characterized by severe hypoglycemia, hyperinsulinemia, insulin antibody (IAA) positive, and never received exogenous insulin therapy. The onset of IAS hypoglycemia was self-limiting, and 82% resolved spontaneously within 1 year without treatment, but the attack was more serious and difficult to diagnose. In addition to symptomatic treatment, the use of adrenocortical hormone may have some value. Genetic susceptibility may play an important role in the development of IAS. Positive results may be diseases: Pregnancy diabetes, type II diabetes precautions The insulin-dependent diabetes anti-islet cell antibody positive rate was 20% to 40%, the active positive rate was 60% to 70%; the non-insulin-dependent diabetes positive rate was only 6.2%. Therefore, detection of anti-islet cell antibodies can distinguish between type 2 diabetes. Inspection process (1) Enzyme-linked immunosorbent assay: The insulin-ovalbumin was diluted to 20 μg/ml with a coating solution, and coated with micropores of a polystyrene reaction plate, 100 μl per well, at 4 ° C overnight. The washing solution was washed 3 times for 3 min each time, and blocked with pH 7.4, 0.01 mol/L PBS (containing 15% calf serum), and 43 ° C for 1 h. After the same method, the serum to be tested (1:100) or negative, positive control, 100 μl per well, 43 ° C for 1 h (37 ° C 2 h). HRP-SPA with the optimum dilution after washing with the same method, 100 μl per well, 45 ° C for 1 h (37 ° C 2 h). After the same method, the substrate (OPD-H2O2) solution was added, and 100 μl per well was developed at 37 ° C for 10 min. The reaction was stopped with 2 mol/L of H 4 SO 4 . The absorbance value at 492 nm was measured by an enzyme-linked immunosorbent assay. (2) Immunoenzyme spot method: The NC membrane was cut into small squares of 5 mm × 5 mm, immersed in pH 7.4, 0.01 mol/L PBS for 30 min, and the filter paper was blotted dry. 2 μl of insulin-ovalbumin cross-linking was spotted in the center of a small square and dried naturally at room temperature. The cells were blocked with pH 7.4, 0.01 mol/L PBS (containing ovalbumin 10.0 g/L), and dried at 45 ° C for 1 h. 2 μl of the serum to be tested was added to the antigen coating, and after 30 minutes at 45 ° C, the cells were washed 3 times with pH 7.4, 0.01 mol/L PBS for 3 min each time, and the filter paper was blotted dry. The membrane was placed in an optimal concentration of HRP-SPA at 45 ° C for 30 min, and washed with the filter paper as above. Immerse in the new formulation for 5-10 minutes, rinse with water after color development, and terminate the reaction. Not suitable for the crowd There are no special taboos. Adverse reactions and risks There are no related complications and hazards.

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