Anti-epidemic hemorrhagic fever virus antibody (EHF-AB)

After the human body is infected with the epidemic hemorrhagic fever virus, EHF-IgM can be detected in the serum from 2 to 3 days after the onset of the disease, reaching a peak in 7 to 10 days. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: Positive epidemic hemorrhagic fever. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. Normal value EHF-IgM negative, EHF-IgG negative. Clinical significance Positive epidemic hemorrhagic fever. Precautions First, the precautions before blood draw: 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the medical examination, you should start fasting for 12 hours to avoid affecting the test results. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, after blood draw should pay attention to: 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. 3. Please inform the doctor about the recent medication and special physiological changes before the test. Inspection process The test serum was diluted with 5 mmol/L PBS (pH 7.2), and then added dropwise to the antigen sheet, and the container was immersed in a humidified box at 37 ° C for 45 minutes. Wash 3 times with PBS, dip for 3 min each time, shake for 2 min, blow dry, add FITC-labeled anti-human IgM prepared with 0.1 g/L Evans Blue-PBS, incubate with the same method, wash and dry, use non-fluorescent polyethylene Alcohol-glycerol gel-filled tablets were observed under a fluorescence microscope. Positive or spot-like fluorescence in the cytoplasm was positive. According to the number of positive cells and fluorescence intensity (+) ~ (++++), positive The reciprocal of the highest dilution of serum is the antibody titer in the test specimen. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.

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