Hepatitis D Virus (HDV)

Hepatitis D virus (HDV) is a defective virus that is enveloped by HBV envelope proteins and cannot replicate itself or proliferate alone. The mode of transmission is mainly through blood transfusion or blood products, and can also be transmitted through close contact with vertical infection between mother and child. High-risk groups include drug addicts and multiple recipients. Basic Information Specialist classification: Infectious disease examination and classification: immunological examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Did not suffer from hepatitis D. Positive: Prompt that there may be viral hepatitis. Tips: Be an empty stomach before checking. Fasting certain foods. The day before the liver function is checked, it is best not to eat foods rich in carotene and lutein. Normal value Anti-HDIgM is elevated. Anti-HDIgG positive. Clinical significance Detection of anti-HDIgM can be used as an indicator to identify HDV and HBV combined or overlapping infections. In the case of co-infection, anti-HDIgM was transiently elevated; in the case of overlapping infections, the increase in anti-HDIgM was mostly persistent, such as an increase in anti-HDIgM accompanied by abnormal liver function, suggesting a worsening of the condition. As for anti-HD IgG positivity, it is generally considered to be a previous infection. Precautions (1) Fasting before inspection. Generally check the blood function and blood lipids when checking liver function. You must not eat before the test, because some substances in the food are absorbed into the blood through the digestive tract after eating, so that the blood components will change, such as blood sugar, chloride and lipids will increase; After the blood fat content will temporarily increase, so that the serum becomes white translucent or chyle, the accuracy of the test results. Under the action of glucocorticoids, the fasting blood glucose of the normal body begins to rise after 6 o'clock in the morning, and reaches the peak at 8 o'clock in the morning. At this time, the situation in the body cannot be truly reflected. Therefore, the blood test should be taken at 6 o'clock in the morning on an empty stomach. For example, in an emergency patient or a patient on a remote road, if necessary, blood can be taken after 6 hours of eating, because the blood will gradually clear out (ie, the serum is restored to its original state), but the blood lipid test should be performed 12 hours after fasting. (2) Forbidden to take certain drugs. Taking medication may affect the accuracy of liver function tests. Such as: taking vitamin D, adipine, chlortetracycline, nitrofurazone and other drugs will affect the determination of jaundice index: taking opioids can lead to increased transaminase activity; with antipyrine, sulfonamides, Drugs such as aminosalicylic acid (PAS), procaine, and urotropine can block the determination of urinary bile cell response. The use of PAS can increase the phenolic quaternary tetrabromide iodate retention test (BSP). (3) Fasting certain foods. The day before the patient's liver function is checked, it is best not to eat foods rich in carotene and lutein, because these foods will make the serum yellow, affecting the determination of the jaundice index; high-fat foods such as fritters, milk, chocolate, Egg meat products, etc., can significantly increase the blood finger test, so it is necessary to ban the consumption of finger foods 10 hours before the blood draw. Inspection process The artificially synthesized HDAg is coated on the microplate, and the serum to be tested is reacted with it, and then the antibody is detected by the enzyme-labeled anti-human IgG, and the substrate is colored, and 50 μl of the serum to be tested is added per well, and a positive and negative control are set. Wash at 37 ° C for 1 h. HPR-goat anti-human IgG was added, 50 μl per well, and washed at 37 ° C for 1 h. The TMB-H2O2 substrate was added for color development at room temperature for 15 min. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.

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