gamma-interferon

Type II interferons, also known as gamma-IFN or immunointerferons, are produced by mitogen-stimulated T lymphocytes. Interferon is a highly effective antiviral biologically active substance and a lymphokine with extensive immunomodulatory effects. There are many methods for detecting interferon, and the currently used enzyme-linked immunosorbent assay (ELISA) is abbreviated as ELISA. Basic Information Specialist classification: Oncology examination classification: immune examination Applicable gender: whether men and women apply fasting: not fasting Tips: Before the examination, the diet is light and alcohol is prohibited. Check for an empty stomach in the morning. Normal value The ELISA method was 1.21 to 5.51 μg/L. Clinical significance Reduce gastric cancer and intestinal cancer. Precautions (1) Microplates must be treated strictly, otherwise it will affect the adhesion growth of L929 cells. (2) The dissolved VSV solution should not be stored at 40 ° C or room temperature for a long time due to inactivation. (3) Mouse spleen cell IFN induction method The mice were sacrificed by cervical necking, the spleen was aseptically taken, and the spleen was ground with a ground glass (or syringe core), allowed to stand for 5 min, and the supernatant was adjusted to a cell concentration of 2×107/ml. . Add 2 ml of ConA (10 μg/ml) cell suspension in a 24-well plate and set without ConA. After incubation for 48 h at 37 ° C in 5% CO 2 , the supernatant (containing IFN) was taken for testing. Inspection process Same as ELISA. Not suitable for the crowd There are no taboos. Adverse reactions and risks There are no related complications and hazards.

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