Urine hemosiderin qualitative test

The urinary hemosiderin qualitative test is an auxiliary test for checking whether urine is normal. A type of urine test. This is a qualitative test for the diagnosis of intravascular hemolysis, mainly used to determine paroxysmal nocturnal hemoglobinuria. When the urine contains hemosiderin, the high iron ion acts on the ferrocyanide, and the blue iron ferrocyanide precipitate in the acidic environment is called the Prussian blue reaction. Through this examination, the corresponding symptoms can be judged. Basic Information Specialist classification: urinary examination classification: urine / kidney function test Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: normal. Positive: No relevant information. Tips: Do not eat after 9:00 pm the day before the test. Drink water. Check the morning after getting up and do not eat or drink water, which is convenient for inspection. Normal value The urinary hemosiderin qualitative test for healthy people in this method is negative. Clinical significance Abnormal results: When hemolysis occurs in the blood vessels, very little hemoglobin is released, and these free hemoglobins are absorbed at the proximal end of the renal tubules and converted into heme and globin. Heme can be converted to heme, which can be excreted in the urine. Therefore, when a positive result occurs, it can be considered that a hemolysis problem occurs in the blood vessel. This test was positive in paroxysmal nocturnal hemoglobinuria. Positive results may be diseases: paroxysmal cold hemoglobinuria, hemolytic anemia in children, idiopathic hemosiderosis, autoimmune hemolytic anemia considerations Taboo before the test: Do not eat after 9:00 pm the day before the test. Drink water. Check the morning after getting up and do not eat or drink water, which is convenient for inspection. Requirements for inspection: The doctor patiently operates and checks carefully. Inspection process 1. Centrifuge 15 ml of fresh urine (5 minutes at 2000 r/min) and discard the supernatant. 2. Add freshly prepared 2% potassium ferrocyanide reagent and 2 ml of 3% hydrochloric acid to the sediment, mix thoroughly, and let stand at room temperature for 10 minutes. 3. Centrifuge again, take the smear of the sediment, and cover with a high power microscope (if necessary, use an oil mirror). 4. If there are scattered or piles of blue glitter particles (1 to 3 μm in diameter), it is positive, as is true in cells. Not suitable for the crowd Inappropriate people: Generally, there is no suitable for the crowd. Adverse reactions and risks No obvious complications and harms.

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