Heat dissipation test

The heat release test is a method for removing antibodies from red blood cells by using thermal effects. It can be used for identification of ABO subtypes, diagnosis of neonatal hemolytic disease, and antibody specificity identification of red blood cells in patients with autoimmune hemolytic anemia, and antibody after absorption. It is then released for identification or preparation of monospecific antibodies. Basic Information Specialist classification: Infectious disease examination and classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: It is generally normal. Positive: Can be seen in neonatal hemolytic disease and autoimmune hemolytic anemia. Tips: Pay attention to rest and ban smoking. Normal value No abnormal antibodies were found in the dispersion results and were negative. Clinical significance Abnormal result The antibodies dispersed from the experiment can be used to diagnose the specificity of ABO subtypes, the antibody specificity of erythrocytes in neonatal hemolytic disease and autoimmune hemolytic anemia patients. Need to check the crowd: blood disease patients, newborns. Positive results may be diseases: neonatal hemolytic disease, autoimmune hemolytic anemia considerations Inappropriate crowd: People with a small number of red blood cells. Taboo before the test: pay attention to rest, no smoking. Requirements for examination: Newborns are sensitive to pain and need to be appeased during blood draw. Strict attention to temperature and time. If the temperature is too high, the cells are easily dissolved. If the temperature is too low, the antibody is not completely dispersed. Inspection process 1. Take 1 part of the washed packed red blood cells and add 1 part of saline. 2. Mix and place in a water bath at 56 ° C for about 10 minutes, and shake them frequently. 3. High speed centrifugation, if possible, use a preheated centrifuge cup. 4. Separate the supernatant. Detect the analysis results. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. 3. People with a small number of red blood cells. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.

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