tissue plasminogen activator

Tissue plasminogen and cargo are single-chain glycoproteins, which are mainly synthesized, secreted and continuously released into the blood by vascular endothelial cells. They are widely present in various tissues of the body, and the liver is inactivated by tissue plasminogen activator. The main place. It has a high affinity for fibrin, then forms plasminogen with tyrosine plasminogen, degrades fibrin (original) and partial coagulation factors, and is a key substance in the fibrinolytic system. Lowering suggests a decrease in fibrinolytic activity. Found in prethrombotic state and thrombotic diseases, such as arterial thrombosis, deep vein thrombosis, ischemic stroke. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: It suggests a decrease in fibrinolytic activity, seen in prethrombotic states and thrombotic diseases. Normal value: Normal value: 0.3-0.5u/mL Above normal: It suggests that fibrinolysis is hyperactive and is seen in primary and secondary fibrinolysis. negative: Positive: Tips: oral contraceptives, strenuous exercise, etc. can increase tissue plasminogen activator. Normal value 0.3 to 0.5 u/mL. (chromogenic substrate method) Clinical significance Lowering suggests a decrease in fibrinolytic activity. Found in prethrombotic state and thrombotic diseases, such as arterial thrombosis, deep vein thrombosis, ischemic stroke. Elevated suggestive of fibrinolytic activity, seen in primary and secondary fibrinolysis, such as disseminated intravascular coagulation, acute promyelocytic leukemia, liver disease, coronary heart disease, hyperlipidemia, stress response. Low results may be diseases: high thrombosis results may be diseases: high blood lipids, coronary heart disease, diffuse intravascular coagulation considerations 1, oral contraceptives, strenuous exercise, etc. can increase tissue plasminogen activator. 2. Tissue plasminogen activator has a tendency to increase with age. Inspection process Antigen assay (t-PA: Ag) ELISA: 1 Dilute the t-PA antibody with 0.05 mol/L carbonate buffer. 2 Dilute the antibody-POD conjugate with dilution buffer. 3 Dissolve o-phenylenediamine (8 mg/ml) with matrix buffer and add 10 μl of 30% H 2 O 2 . 4 The t-PA standard was diluted to 10, 5, 2.5, 1.25, 0.625, and 0.3125 IU/ml. 5 specimen dilution: 1 part sodium citrate anticoagulated plasma plus 5 dilutions. If the t-PA value is estimated to increase, the plasma is diluted 1:10. 6 The ELISA plate was coated with diluted t-PA antibody, 200 μl per well, incubated overnight, and then washed 3 times with washing solution. 7 plus standard / specimen 200μl, after 1h incubation, wash the same as 3 times. 8 plus 20 μl of peroxidase antibody complex, after 1 h of incubation, wash 3 times as above, and add the matrix immediately after washing. 9 Add 200 μl of matrix to each well and develop for 10-15 min. 10 plus sulfuric acid (3mol / L) 50μl or hydrochloric acid (1mol / L) 100μl, 10min suspension reaction, colorimetric at 492nm, 2h, with a dilution buffer background. 11 According to the absorbance reading, the t-PA content was found from the standard curve. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.

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