Autohemolysis test and correction test

Under normal conditions, normal human heparin anticoagulation is placed in the environment of 37 ° C for 24-48 hours, the red blood cells are finally destroyed due to energy consumption, that is, slight hemolysis occurs. When glucose, adenosine triphosphate (ATP) is added, different degrees of correction can be obtained. The trial is a more sensitive test for screening and identifying hemolytic anemia due to defects in erythrocyte membranes. This test is mainly used for the etiological diagnosis of hemolytic anemia. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Normal value: no Above normal: negative: Generally it is normal. Positive: More common in diseases such as hemolytic anemia. Tips: Do not eat too greasy, high-protein foods the day before the blood draw, avoid heavy drinking. Normal value Qualitative test: negative. Quantitative test In physiological saline: hemolysis rate <0.005 at 24h, hemolysis rate <0.035 at 48h. Add 10% glucose: 24h hemolysis rate <0.004, 48h hemolysis rate <0.006. Add ATP: visible hemolysis at 24h, no hemolysis for 48h. Clinical significance This test is mainly used for the etiological diagnosis of hemolytic anemia. 1, hereditary spherocytosis is significantly increased, and can be corrected with glucose and ATP. 2. Other hereditary non-spherical erythrocyte hemolytic anemia can also be increased and corrected by glucose or ATP, respectively. 3, pyruvate kinase deficiency, autoimmune hemolytic anemia, paroxysmal nocturnal hemoglobinuria, drug-induced hemolysis, etc., plus glucose can not be corrected, plus ATP can be corrected. Positive results may be diseases: hemolytic anemia, pediatric hereditary spherocytosis considerations First, the precautions before blood draw 1, do not eat too greasy, high-protein food the day before the blood, to avoid heavy drinking. The alcohol content in the blood directly affects the test results. 2. After 8 pm on the day before the physical examination, fasting should be done to avoid affecting the detection of indicators such as blood glucose in the second sky. 3, should relax when taking blood, to avoid the contraction of blood vessels caused by fear, increase the difficulty of blood collection. Second, should pay attention after blood draw 1. After blood is drawn, local compression is required at the pinhole for 3-5 minutes to stop bleeding. Note: Do not rub, so as not to cause subcutaneous hematoma. 2, the pressing time should be sufficient. There is a difference in clotting time for each person, and some people need a little longer to clotting. Therefore, when the surface of the skin appears to be bleeding, the compression is stopped immediately, and the blood may be infiltrated into the skin due to incomplete hemostasis. Therefore, the compression time is longer to completely stop bleeding. If there is a tendency to bleed, the compression time should be extended. 3, after the blood draw symptoms of fainting such as: dizziness, vertigo, fatigue, etc. should immediately lie down, drink a small amount of syrup, and then undergo a physical examination after the symptoms are relieved. 4. If there is localized congestion, use a warm towel after 24 hours to promote absorption. Inspection process The red blood cells were placed in a sucrose solution with a low ion concentration, and at 37 ° C, the binding of complement and red blood cells was promoted, and whether the red blood cells were destroyed or not hemolysis was observed. Not suitable for the crowd 1. Patients who have taken contraceptives, thyroid hormones, steroid hormones, etc., may affect the results of the examination and prohibit patients who have recently taken the drug history. 2, special diseases: patients with hematopoietic function to reduce disease, such as leukemia, various anemia, myelodysplastic syndrome, etc., unless the examination is essential, try to draw less blood. Adverse reactions and risks 1, subcutaneous hemorrhage: due to pressing time less than 5 minutes or blood draw technology is not enough, etc. can cause subcutaneous bleeding. 2, discomfort: the puncture site may appear pain, swelling, tenderness, subcutaneous ecchymosis visible to the naked eye. 3, dizzy or fainting: in the blood draw, due to emotional overstress, fear, reflex caused by vagus nerve excitement, blood pressure decreased, etc. caused by insufficient blood supply to the brain caused by fainting or dizziness. 4. Risk of infection: If you use an unclean needle, you may be at risk of infection.

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