serum ferritin

Ferritin is one of the main forms of iron stored in the human body. It has the ability to combine iron and reserve iron to maintain the body's iron supply and the relative stability of hemoglobin. Serum ferritin is the most sensitive indicator for detecting iron deficiency in the body. It is used to diagnose iron deficiency anemia, liver disease, etc. It is also one of the markers of malignant tumors. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Analysis results: Below normal: Found in iron deficiency anemia, nutritional anemia and so on. Normal value: Male: 15-200μg/L Female: 12-150μg/L Newborn: 25-200μg/L Above normal: The source of ferritin increases or clears the barrier. negative: Positive: Tips: Before using the iron, use iron to treat or fast foods with high iron content, such as animal liver, fasting and iron complexing substances, such as tea. Normal value Male 15-200 μg / L. Female 12-150μg / L. Newborns 25-200μg / L. Because the reagents and methods are not standardized, each laboratory must have its own reference value. Clinical significance 1. The reason for the increase in ferritin is that the source of ferritin is increased or cleared. Such as excessive blood transfusion, malnutrition, inflammation, liver disease and so on. Suffering from malignant tumors such as liver cancer, lung cancer, pancreatic cancer, leukemia, etc., especially primary liver cancer, the increase in ferritin synthesized by cancer cells increases serum ferritin. 2, ferritin reduction found in iron deficiency anemia, nutritional anemia, blood loss, long-term diarrhea caused by iron absorption disorders, infection, cirrhosis and so on. Low results may be diseases: leukemia, pregnancy with iron deficiency anemia, iron utilization anemia high results may be diseases: premature infant anemia, restless legs syndrome precautions 1. Serum iron is highest in the morning in 1 day and gradually decreases in the afternoon. Blood samples should be taken on an empty stomach in the morning. 2, before the examination, use iron to treat or fast foods with high iron content, such as animal liver, fasting energy and iron complex substances, such as tea. 3, when women in the menstrual period, pregnancy and infant growth period, due to the increase in the body's iron requirements, serum iron can be reduced, is a physiological phenomenon. Inspection process Take a clean, dried polystyrene microplate reaction plate as follows. (1) 100 μl of anti-ferritin serum was added to each well of the assay, standard and blank, placed at 4 ° C overnight, and each well was washed 3 times with washing solution, and each time was allowed to stand at room temperature for 3 min. (2) Add 100 μl of series ferritin standard solution and sample (diluted 10 times with dilution solution) in the standard and measuring wells, set at 37 ° C for 50 min, and wash each well 3 times with 9 g/L NaCl, and wash as above. (3) 100 μl of enzyme-labeled antibody was added to each well, placed at 37 ° C for 50 min, and washed 3 times with 9 g / L NaCl. (4) 100 μl of the substrate solution was added to each well, and the color was developed at 37 ° C for 30 min. (5) Finally, 50 μl of 2 mol/L H 2 SO 4 was added to each well to terminate the reaction, and the absorbance of each well was read by colorimetry at 492 nm. Note: (1) Avoid excessive force during the washing process to prevent the conjugate adsorbed on the polystyrene from being washed away. (2) The polyethylene test tube method can be used. At this time, the amount of the reagent should be appropriately increased, and finally the color is measured by a spectrophotometer. Not suitable for the crowd A patient with hemophilia and severe clotting factor deficiency. Adverse reactions and risks Risk of infection: If you use an unclean needle, you may be at risk of infection.

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