Red blood cell incubation osmotic fragility test

The erythrocyte incubation osmotic fragility test is to detect the fragility of human macro cells after incubation, and is used for the diagnosis and differential diagnosis of hereditary non-spherical erythrocyte hemolytic anemia. Basic Information Specialist classification: cardiovascular examination classification: blood examination Applicable gender: whether men and women apply fasting: fasting Tips: Pay attention to rest before the examination, and keep blood on an empty stomach. Normal value Colorimetric method, adult 65-100 negative, infant (umbilical blood) 55-100 negative. Sodium chloride method erythrocyte intermediate brittleness 4.65 ~ 5.90g / L sodium chloride solution. Clinical significance Abnormal results: Erythrocyte incubation osmotic fragility test, red blood cells in hypotonic saline solution, when water penetrates into the interior to a certain extent, red blood cells expand and rupture. Observing the degree of hemolysis of red blood cells in different concentrations of salt solution, it can be judged that it is resistant to hypotonic saline solution. This resistance is related to the ratio of surface area to volume of red blood cells. The smaller the ratio, the less red blood cell resistance and the more brittleness. On the contrary, the resistance increases. The detection method is colorimetric method, the result is less than 65%, the intermediate brittleness of sodium chloride method is less than 4.65~5.90g/L sodium chloride solution, and may have mild hereditary polycythemia, hereditary non-spherical erythrocyte hemolytic anemia. The people who need to be examined have a family history of blood diseases and an anemia population. Precautions Inappropriate crowd: None. Taboo before the test: pay attention to rest, keep blood on an empty stomach. Do not wear clothes that are too small or too tight in cuffs to avoid the sleeves being too tight when blood is drawn or the sleeves are too tight after blood draw, causing blood vessels in the arms. Avoid strenuous exercise. Requirements for examination: Do not tamper with venous blood collection, check is cumbersome, it may take a long time, you need to wait patiently for results. Inspection process Vascular blood collection was used for testing. Before venous blood collection, carefully check that the needle is securely installed and that there is air and moisture in the syringe. The needle used should be sharp, smooth, ventilated, and the syringe should not leak. Firstly, the skin was disinfected from the inside to the outside and clockwise from the selected venipuncture with a 30g/L iodine swab. After the iodine was volatilized, the iodized trace was wiped out in the same way with a 75% ethanol swab. Fix the lower end of the venipuncture site with the thumb of the left hand, hold the syringe syringe with the thumb and middle finger of the right hand, and fix the needle lower seat with the index finger, so that the bevel of the needle and the scale of the syringe are upward, and the needle is inclined along the vein to make the needle and the skin obliquely penetrate the skin at an angle of 30°. Then, through the vein wall, enter the venous cavity forward at an angle of 5°. After seeing the blood return, the needle will be probed into the spot to avoid the needle slipping out when the blood is collected. However, it is not possible to use a deep puncture to avoid hematoma, and immediately remove the cuff. Needle plug can only be pumped out, can not be pushed in, so as to avoid injecting air into the vein to form a gas plug, causing serious consequences. Remove the syringe needle and slowly inject the blood into the anticoagulation tube along the tube wall to prevent hemolysis and foam. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Discomfort: There may be pain, swelling, tenderness, and visible subcutaneous ecchymosis at the puncture site.

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