Acetate AS-D tea phenol lipase staining

Chloroacetate naphthol esterase is a marker enzyme for granulocytes and mast cells. It is mainly present in lysosomes of granulocytes. This enzyme is positively reacted with peroxidase in neutrophils, but the product formed by this enzyme is more than Oxidase is more specific. Helping to identify acute leukemia of acute leukemia type, leukemia granulocytes can be positive, acute leukemia and acute lymphoblastic leukemia, leukemia cells were negative. Basic Information Specialist classification: growth and development examination classification: blood examination Applicable gender: whether men and women apply fasting: not fasting Analysis results: Below normal: Normal value: no Above normal: Above normal, it is easy to cause blood diseases. negative: In acute monocytic leukemia and acute lymphocytic leukemia, leukemia cells are negative. Positive: To help identify acute leukemia type acute myeloid leukemia, leukemia granulocytes can be positive. Tips: Strictly follow the operating procedures, such as increasing temperature and pH can lead to the decomposition of chloroacetate, which produces a distinct non-specific esterase reaction in the cell. Normal value The granulocyte system is a negative reaction of protoplasts, which is positive from promyelocytes to mature neutrophils, and the enzyme activity does not increase with cell maturation. Mast cells are positive. Monocytes, lymphocytes, plasma cells, young red blood cells, and platelet cells all had negative reactions. Clinical significance Helping to identify acute leukemia of acute leukemia type, leukemia granulocytes can be positive, acute leukemia and acute lymphoblastic leukemia, leukemia cells were negative. High results may be diseases: Pregnancy with leukemia, secondary leukemia considerations (1) The optimal reaction pH of naphthol esterase (NCE) of chloroacetate is between 7.0 and 7.6. It is not inhibited by sodium fluoride. (2) Strict adherence to the operating procedures, such as increased temperature and pH can lead to the decomposition of chloroacetate, which produces a distinct non-specific esterase reaction in the cell. (3) In addition to the new infrared method used in this law, some people choose strong purple or solid blue. Inspection process (1) Fixation: freshly dry smear, fixed with formaldehyde-methanol fixing solution for 30s, or with formaldehyde vapor for 5~10min. (2) Washed and dried. (3) Put in the working solution, 37 ° C for 30 min. (4) Washing, hematoxylin dyeing solution counterstaining for 1 to 2 minutes. (5) Washed, dried, and examined. Not suitable for the crowd Have a coagulopathy such as hemophilia. Adverse reactions and risks Infection: Do not touch the dirt after blood collection. Do not wash your hands immediately to avoid infection.

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